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. 1991 Feb 1;88(3):727–731. doi: 10.1073/pnas.88.3.727

Calcium channels from Cyprinus carpio skeletal muscle.

M Grabner 1, K Friedrich 1, H G Knaus 1, J Striessnig 1, F Scheffauer 1, R Staudinger 1, W J Koch 1, A Schwartz 1, H Glossmann 1
PMCID: PMC50886  PMID: 1846962

Abstract

The complete amino acid sequence of the L-type calcium channel alpha 1 subunit from the carp (Cyprinus carpio) white skeletal muscle was deduced by cDNA cloning and sequence analysis. The open reading frame encodes 1852 amino acids (Mr 210,060). A 155-amino acid COOH-terminal sequence (after the fourth internal repeat) is evolutionarily preserved (90% homology) and may represent an important functional domain of L-type calcium channels. The photolabeled, membrane-bound, and purified carp alpha 1 subunits have masses of 211 and 190 kDa. The purified channel could not be phosphorylated by cAMP-dependent protein kinase. Two glycoproteins (alpha 2 subunits) are associated with the alpha 1 subunit and change their apparent masses from 235 and 220 kDa to 159 kDa upon reduction of disulfide bonds. Nucleic acid hybridization with alpha 2 cDNA revealed an 8.0-kilobase transcript in carp skeletal muscle. Evidence for a copurification of subunits similar in size to mammalian beta or gamma subunits was not obtained.

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Selected References

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