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. 1991 Feb 1;88(3):741–744. doi: 10.1073/pnas.88.3.741

Electron probe microanalysis of calcium release and magnesium uptake by endoplasmic reticulum in bee photoreceptors.

O Baumann 1, B Walz 1, A V Somlyo 1, A P Somlyo 1
PMCID: PMC50889  PMID: 1992466

Abstract

Honey bee photoreceptors contain large sacs of endoplasmic reticulum (ER) that can be located unequivocally in freeze-dried cryosections. The elemental composition of the ER was determined by electron probe x-ray microanalysis and was visualized in high-resolution x-ray maps. In the ER of dark-adapted photoreceptors, the Ca concentration was 47.5 +/- 1.1 mmol/kg (dry weight) (mean +/- SEM). During a 3-sec nonsaturating light stimulus, approximately 50% of the Ca content was released from the ER. Light stimulation also caused a highly significant increase in the Mg content of the ER; the ratio of Mg uptake to Ca released was approximately 0.7. Our results show unambiguously that the ER is the source of Ca2+ release during cell stimulation and suggest that Mg2+ can nearly balance the charge movement of Ca2+.

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Selected References

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