Prevalence and Clinicopathological Features of Triaditis in a Prospective Case Series of Symptomatic and Asymptomatic Cats

FC Fragkou; KK Adamama‐Moraitou; T Poutahidis; NN Prassinos; M Kritsepi‐Konstantinou; PG Xenoulis; JM Steiner; JA Lidbury; JS Suchodolski; TS Rallis

doi:10.1111/jvim.14356

. 2016 Jun 14;30(4):1031–1045. doi: 10.1111/jvim.14356

Prevalence and Clinicopathological Features of Triaditis in a Prospective Case Series of Symptomatic and Asymptomatic Cats

FC Fragkou ^1,^✉, KK Adamama‐Moraitou ¹, T Poutahidis ², NN Prassinos ³, M Kritsepi‐Konstantinou ⁴, PG Xenoulis ^5,^6,⁷, JM Steiner ⁵, JA Lidbury ⁵, JS Suchodolski ⁵, TS Rallis ¹

PMCID: PMC5089651 PMID: 27296565

Abstract

Background

The term triaditis designates the concurrent presence of idiopathic inflammatory bowel disease (IBD), cholangitis, and pancreatitis in cats.

Hypothesis/Objectives

The histopathology of concurrent, but often subclinical, inflammatory processes in the small intestine, liver, and pancreas of cats is poorly described. We aimed to investigate the frequency of enteritis, cholangitis, pancreatitis, or some combination of these in symptomatic and asymptomatic cats, compare clinicopathological features, and correlate histopathological with laboratory findings.

Animals

Domestic cats (27 symptomatic, 20 asymptomatic, and 8 normal).

Methods

Prospective study. Physical examination, laboratory variables (CBC, serum biochemistry profile, serum thyroxine concentration, serum feline trypsin‐like immunoreactivity [fTLI], feline lipase immunoreactivity [fPLI, as measured by Spec fPL ^®], urinalysis, and fecal analysis), imaging, and histopathological examinations were conducted. Feline liver, pancreas, and small intestine were biopsied during laparotomy.

Results

Inflammatory lesions were detected in 47 cats (27 symptomatic, 20 asymptomatic). In total, 20 cats had histopathologic lesions of IBD (13/47, 27.7%), cholangitis (6/47, 12.8%), or pancreatitis (1/47, 2.1%) alone, or inflammation involving >1 organ (27/47, 57.4%). More specifically, 16/47 cats (34.0%) had concurrent lesions of IBD and cholangitis, 3/47 (6.4%) of IBD and pancreatitis, and 8/47 cats (17%) of triaditis. Triaditis was identified only in symptomatic cats (8/27, 29.6%). A mild, positive correlation was detected between the severity (score) of IBD lesions and the number of comorbidities (rho = +0.367, P = .022).

Conclusions and Clinical Importance

Histopathological evidence of IBD or IBD with comorbidities was detected in both symptomatic and asymptomatic cats. The possibility of triaditis should be considered in symptomatic cats with severe IBD.

Keywords: Cat, Cholangitis, Inflammatory Bowel Disease, Pancreatitis

Abbreviations

Ch: cholangitis
P: pancreatitis
IBD: idiopathic inflammatory bowel disease
fTLI: feline trypsin‐like immunoreactivity
fPLI: feline pancreatic lipase immunoreactivity
FIV: feline immunodeficiency virus
FeLV: feline leukemia virus
ALB: albumin
BUN: blood urea nitrogen
Crea: creatinine
ALP: alkaline phosphatase
ALT: alanine amino transferase
γGT: γ‐glutamyltransferase
AST: aspartate transaminase
TBIL: total bilirubin
LIPA: lipase activity
Ca: calcium
P: phosphorus
K: potassium
Na: sodium
PT: prothrombin time
PTT: partial thromboplastine time
T₄: total thyroxine
fT₄: free thyroxine concentrations
95% CI: 95% Confidence Interval

The term triaditis has been coined to describe concurrent inflammatory infiltration of the intestines (IBD), the biliary system, and the pancreas in cats.1, 2 However, the terminology for this condition remains controversial. The controversy over the characterization of triaditis as a separate syndrome is not surprising, considering that the 3 component diseases remain poorly characterized with regard to their pathogenesis and their clinical, laboratory, and histopathological manifestations.2 In addition, the inconsistent clinical and laboratory findings and lack of specific and sensitive laboratory tests often make their ante‐mortem diagnosis challenging. Histopathologic evaluation of hepatic, pancreatic, and intestinal biopsy specimens is essential for a definitive diagnosis. However, no consensus exists regarding the histopathological nomenclature and classification of the lesions of the component diseases of triaditis. Also, interpretation of borderline lesions of these component diseases might be challenging. These factors have made it difficult to compare results of different studies and definitively define clinical, diagnostic, and therapeutic standards. To address this problem, the World Small Animal Veterinary Association (WSAVA) has formed the Liver Standardization Group,3 and later, the Gastrointestinal Standardization Group,4 which have summarized histopathological standards for the diagnosis of hepatic and gastrointestinal inflammation in biopsy samples from dogs and cats.3, 4, 5 However, since the publication of these standards in 2006 and 2010,5, 6 there have been concerns about the validity and reproducibility of these standards in clinical cases.7

To date, there is little literature focused on feline triaditis and clinical studies are lacking. Several case reports describe cats with different combinations of inflammatory conditions of the intestine, liver, and pancreas, in some patients also in combination with hepatic lipidosis, chronic nephritis, or some combination of these disorders.2, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 However, these reports are either incomplete in terms of laboratory analysis22, 23, 24 or include only a small number of cases.21, 25, 26 Current knowledge mostly is derived from retrospective postmortem studies.1, 27, 28, 29, 30, 31 In 2 independent studies, the coexistence of feline cholangiohepatitis or cholangitis with pancreatitis and IBD was reported in 39%29 or 32%31 of cats examined.

Clinical studies using adequate numbers of cats with complete clinical and laboratory analyses would facilitate understanding of the enigmatic nature of feline triaditis. Our study aimed primarily to investigate the frequency of coexisting permutations of IBD, cholangitis and pancreatitis in symptomatic and asymptomatic cats, and describe histopathological, clinical, and laboratory features. Secondarily, comparisons between different combinations of inflammatory lesions were made and correlations between histopathological and laboratory variables were investigated.

Materials and Methods

Study Design

This prospective study involved domestic cats admitted to the Companion Animal Clinic of the School of Veterinary Medicine, Aristotle University of Thessaloniki (February 2008–February 2011). The protocol was approved by the School of Veterinary Medicine (Approval No: 435/12‐2‐2008) and licensed by the National Veterinary Administration authorities (License No: 13/3657/29‐03‐2010). Two categories of cats were evaluated for the purpose of this study: symptomatic cats presented to the Companion Animal Clinic with chronic clinical signs that could be attributed to triaditis (persistent or recurrent lethargy, increased or decreased appetite, vomiting, abnormal feces, jaundice, weight loss, or some combination of these signs), as well as asymptomatic cats presented for ovariohysterectomy.

Inclusion criteria for enrollment into the study were as follows: (1) minimum age of 1 year; (2) consumption of commercial cat food (dry, canned, or both) for at least 8 consecutive weeks before initial examination; (3) written consent from the cat owner; and (4) histopathological evidence of enteritis, pancreatitis, cholangitis, or some combination of these, with or without compatible clinical signs at the time of examination (study group) or normal clinical and histopathological findings (control group).

Exclusion criteria were as follows: (1) any drug use such as antibiotics, anti‐inflammatory drugs, or immunosuppressant drugs, in the last 2 weeks before the examination; (2) presence of clinical or laboratory findings or both of other diseases that could affect the feline liver, pancreas, or small intestine; (3) positive results of parasitological fecal examination; (4) positive test results for feline immunodeficiency virus (FIV), feline leukemia virus (FeLV), feline coronavirus, or some combination of these; (5) abnormal serum total or free T4 concentrations or both; and, (6) presence of diagnostic imaging, histopathological findings, or both compatible with neoplasia, or with inflammatory or noninflammatory lesions other than those investigated for the purpose of this study.

Symptomatic Cats

During the study period, 302 cats with clinical signs were evaluated, of which 82 fulfilled the clinical inclusion criteria for enrollment, but for 43 cats the owners did not consent to the collection of biopsy specimens. Thus, 39 symptomatic cats were fully investigated, several of which were excluded from the study because of absence of histopathological evidence of enteritis, cholangitis, pancreatitis, or some combination of these on histopathological examination.

Asymptomatic Cats

During the same period, 39 cats without any clinical signs of disease also were fully investigated, using the same diagnostic procedures as those used in the symptomatic cats. Biopsy specimens were collected during a scheduled ovariohysterectomy after the cat owners had given written consent. After completion of histopathological examination, a number of cats were excluded from the study because of the specific criteria.

After histopathological examination, all cats with inflammatory lesions detected in the organs examined were included in the study group regardless of the presence of clinical signs (and therefore the study group consisted of cats with histopathological evidence of inflammation in the intestine, liver, pancreas, or some combination of these, with or without clinical signs), whereas asymptomatic cats with normal histopathological findings in the intestines, liver, and pancreas constituted the control group.

Clinical Examination and Diagnostic Testing

History, signalment, and physical examination findings were recorded for all 78 cats examined. Laboratory analysis (<3 days before laparotomy) for all 78 cats included fecal parasitological examination, CBC,¹ serum biochemistry profile: albumin (ALB),² blood urea nitrogen (BUN),² creatinine (Crea),² alkaline phosphatase activity (ALP),² alanine amino transferase activity (ALT),² γ‐glutamyl transferase activity (γGT),² aspartate transaminase activity (AST),² total bilirubin (TBIL),² lipase activity (LIPA),² calcium (Ca),² phosphorus (P),² potassium (K),³ sodium (Na),³ blood coagulation profile including prothrombin time (PT)⁴ (76/78) and partial thromboplastine time (PTT)⁴ (76/78), serum total thyroxine (T₄) concentration,⁵ free thyroxine (fT₄) concentration,⁵ serum feline pancreatic lipase immunoreactivity (fPLI measured by Spec fPL^® ⁶ )32, 33, 34, 35 (76/78), trypsin‐like immunoreactivity (fTLI⁷ ; 77/78), and FIV,⁸ FeLV,⁸ and feline coronavirus⁹ testing. Diagnostic imaging included thoracic and abdominal radiographs¹⁰ (69/78) and abdominal ultrasonography¹¹ (76/78).

Histopathology

A minimum of 5 tissue samples, 1 each from the feline liver, pancreas, duodenum, jejunum, and ileum were collected during surgery from all cats examined (Data S1). Intestinal full‐thickness biopsy specimens were collected. An ileal biopsy was not taken from 1 cat of the study group and this cat was excluded from further analysis. The formalin‐fixed biopsy samples were embedded in paraffin, cut at 4 μm, and stained with hematoxylin and eosin. All biopsy samples were evaluated in a blinded fashion by a veterinary pathologist (TP). Cholangitis,3, 6 enteritis,4, 5, 36 and pancreatitis37 were diagnosed based on previously described histopathological criteria,4, 5, 6, 37 and severity was scored semi‐quantitatively on an ascending scale of 0–4. Scores were given on the basis of inflammatory cell accumulation, fibrosis, and epithelial lesions according to the following scale: 0: normal; 1: mild; 2: mild to moderate; 3: moderate; and 4: severe. The highest among the separate scores given to each part of the intestine was considered as the enteritis score. Examples of the grading system of cholangitis, pancreatitis, and enteritis are shown in Figs 1, 2, 3. A cholangitis score of 4, corresponding to portal‐to‐portal bridging lesions, was not assigned to any of the cats examined and thus is not illustrated in Fig. 1. Hepatic lipidosis also was scored on a 0–3 ascending scale based on the extent of hepatic cell lipidosis in the liver sections examined (0: normal; 1: up to 1/3 of hepatocytes affected; 2: up to 2/3 of hepatocytes affected; and 3: all hepatocytes affected).

Histopathological grading of pancreatitis lesions in cats. Score 0 corresponds to the normal histomorphology of the pancreas. Inflammatory cells are gathered mainly at the interlobular spaces. Interlobular fibrosis and replacement of the exocrine pancreas by connective tissue progressively increase in severity from score 1 to 4. Right panel images highlight areas from the images shown on the left at a higher magnification. Bars of left panel images: 100 μm. Bars of right panel images: 50 μm. Hematoxylin‐eosin.

Representative images of the histopathological grading of IBD. Histopathology images of feline ileum are shown. Score 0 corresponds to histomorphologically normal ileum. The severity of chronic enteritis evident by inflammatory cell infiltration of the mucosa and fibrosis of the lamina propria progressively increases from score 1 to 4. Right panel images highlight areas from the images shown on the left at a higher magnification. Bars of left panel images: 100 μm. Bars of right panel images: 50 μm. Hematoxylin‐eosin.

Statistical Analyses

Using histopathological results as a criterion, the cats of the study were classified into groups. Data were processed and comparisons among groups and between symptomatic and asymptomatic cats were made. The cats with pancreatitis lesions alone and IBD lesions combined with pancreatitis were excluded from analysis because of small sample size. Continuous variables were compared by a Kruskal‐Wallis or Mann‐Whitney U‐test, as appropriate, whereas a z‐test was used for comparisons of proportions (percentages [%]; z‐test with Bonferroni correction to the significance level). Differences among median values were highlighted by reporting the minimum statistically significant medians’ difference observed at a significance level P ≤ .05 (MSSDO). In all hypothesis testing procedures, the observed significance level (P‐value) was estimated, as appropriate, either with the exact method (Exact Method) or a Monte‐Carlo simulation based on 10,000 resampling cycles.38 Binomial or bootstrap 95% confidence intervals (CI) were computed for proportions (%). Correlations between the histopathological and laboratory variables were examined with Spearman's rho coefficients. The level of significance was set at P ≤ .05. Statistical analyses were performed by a statistical software package.¹²

Results

Histopathological Examination

Symptomatic cats

Of 39 symptomatic cats examined, 27 ([69.2%], 95% CI: 56.4–79.5%) were diagnosed with inflammatory lesions in at least 1 of the 3 target organs and were included in this study, whereas 12 cats (12/39 [30.8%], 95% CI: 17.9–43.6%) were diagnosed with neoplasms or other noninflammatory conditions (Data S2) and were excluded from this study.

The 27 symptomatic cats of the study group were diagnosed with cholangitis (2/27 [7.4%] cholangitis alone; 95% CI: 0–18.5%), pancreatitis (1/27 [3.7%] pancreatitis alone; 95% CI: 0–11.1%), or IBD (8/27 [29.6%] IBD alone; 95% CI: 14.8–48.1%). Concomitant inflammation in 2 organs (IBD with either cholangitis [6/27] or pancreatitis [2/27]) was evident in 8/27 ([29.6%]; 95% CI: 14.8–48.1%) cats and inflammation in all 3 organs (triaditis) in 8/27 ([29.6%]; 95% CI: 14.8–48.1%) cats.

Asymptomatic Cats

Histopathological examination of 39 asymptomatic cats sampled during ovariohysterectomy, indicated the presence of inflammatory lesions of the liver, pancreas, intestine, or some combination of these in 20 cats ([51.3%]; 95% CI: 38.5–64.1%). Eleven of the asymptomatic cats were removed from the study according to the exclusion criteria (Data S3). The remaining 20 asymptomatic cats with inflammatory lesions and the 27 symptomatic cats with inflammation constituted our study group (47 cats) for further analysis, whereas the 8 remaining clinically and histopathologically normal cats comprised the control group.

Of the 20 asymptomatic cats of the study group, lesions of cholangitis alone were identified in 4/20 ([20%]; 95% CI: 5–40%) cats, enteritis alone in 5/20 ([25%]; 95% CI: 5–45%), and a combination of enteritis and cholangitis (10/20) or enteritis and pancreatitis (1/20) was identified in 11/20 ([55%]; 95% CI: 35–75%) of cats. Neither pancreatitis alone nor triaditis alone was identified among asymptomatic cats.

Assignment of Cats to Groups

Using histopathological evidence of inflammation as a criterion, the 55 symptomatic and asymptomatic cats of the study were further classified into 7 groups as follows: (1) control (Ctrl; n = 8); (2) IBD only (n = 13); (3) cholangitis only (Ch; n = 6); (4) pancreatitis only (P; n = 1); (5) IBD and Ch (IBD+Ch; n = 16); (6) IBD and P (IBD+P; n = 3); and (7) IBD, Ch, and P (IBD+Ch+P; n = 8).

Histopathological Evidence of IBD

Macroscopically, 16 cats had evidence of enteritis at laparotomy, including mild enlargement of the mesenteric lymph nodes (11) or diffuse and mildly firm intestinal wall (7).

Forty of the 47 cats of the study group ([85.1%]; 95% CI: 71.7–93.8%) had histologic evidence of inflammation compatible with IBD; 16/20 ([80%]; 95% CI: 56.3–94.3%) asymptomatic cases with only histologically detected lesions and 24 of the 27 ([88.9%]; 95% CI: 70.8–97.6%) symptomatic cases. In 27 of those 40 cases ([67.5%]; 95% CI: 52.5–80%), concurrent inflammation of the bile ducts, pancreas, or both was detected; cholangitis in 16/40 ([40%]; 95% CI: 27.5–55%), pancreatitis in 3/40 ([7.5%]; 95% CI: 0–17.5%), and triaditis in 8/40 ([20%]; 95% CI: 10–30%).

As for IBD lesions, an abnormal infiltrate of inflammatory cells was present in the lamina propria (Fig 3). The inflammatory cell component primarily consisted of lymphocytes, plasma cells, and macrophages. Neutrophils also were present in variable numbers. In a few cases (2/40), occasional eosinophils also were observed. In the majority of cases, a prominent increase in intraepithelial lymphocytes was observed, whereas in a small percentage of cases intraepithelial globule leukocytes also were evident. Depending on the predominant type of inflammatory cells, IBD lesions were further characterized as lymphocytic, plasmacytic, or lymphoplasmacytic. The architectural lesions of IBD are described in Table 1. The histopathological lesions of IBD extended in all parts (duodenum, jejunum, and ileum) of the small intestine examined with a variable degree of severity.

Table 1.

Architectural lesions associated with idiopathic inflammatory bowel disease (IBD) in symptomatic and asymptomatic cats

Lesions of enteritis	Symptomatic (N = 23)			Asymptomatic (N = 16)
	Score of Lesion			Score of Lesion
	0	1–2	3	0	1–2	3
Submucosal edema, n (%)	7 (30.4%)	14 (60.9%)	2 (8.7%)	6 (37.5%)	9 (56.25%)	1 (6.25%)
Lamina propria fibrosis, n (%)	6 (26.1%)	13 (56.5%)	4 (17.4%)	1 (6.25%)	12 (75%)	3 (18.75%)
Epithelial hyperplasia, n (%)	11 (47.8%)	10 (43.5%)	2 (8.7%)	7 (43.75%)	9 (56.25%)	0 (0%)
Villous hyperplasia, n (%)	19 (82.6%)	2 (8.7%)	2 (8.7%)	14 (87.5%)	2 (12.5%)	0 (0%)
Villous atrophy, n (%)	8 (34.8%)	8 (34.8%)	7 (30.4%)	3 (18.75%)	11 (68.75%)	2 (12.5%)
Microerosions, n (%)	5 (21.7%)	9 (39.1%)	9 (39.1%)	5 (31.25%)	9 (56.25%)	2 (12.5%)
Crypt hyperplasia, n (%)	7 (30.4%)	9 (39.1%)	7 (30.4%)	10 (62.5%)	6 (37.5%)	0 (0%)
Dysplasia, n (%)	19 (82.6%)	4 (17.4%)	0 (0%)	16 (100%)	0 (0%)	0 (0%)

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Score: Score 0: normal; 1: mild, 2: mild to moderate; 3: moderate; 4: severe. The highest among the separate scores assigned to any section of the intestine for each lesion was considered as the overall score for that lesion. Scores 1 and 2 are presented together.

Symptomatic: cats with histopathological findings of IBD and clinical signs.

Asymptomatic: cats with histopathological findings of IBD without clinical signs.

The severity of enteritis of the cats in either the symptomatic or asymptomatic groups is shown in Table 2. There was no significant difference of IBD scores between symptomatic and asymptomatic cats (P = .461).

Table 2.

Medians and ranges (min–max) of the scores of histopathological lesions of idiopathic inflammatory bowel disease (IBD), cholangitis, and pancreatitis in both symptomatic (S) and asymptomatic (A) cats of the study group, divided by subgroup based on organ involvement

Group			IBD Score	Cholangitis Score	Pancreatitis Score
Group		n	Median (Range)	Median (Range)	Median (Range)
Ctrl (n = 8)	A	8	0 (0)	0 (0)	0 (0)
Ctrl (n = 8)	S	0	–	–	–
IBD (n = 13)	A	5	2 (1–3)	0 (0)	0 (0)
IBD (n = 13)	S	8	2 (1–4)	0 (0)	0 (0)
Ch (n = 6)	A	4	0 (0)	1.5 (1–2)	0 (0)
Ch (n = 6)	S	2	0 (0)	2 (2–2)	0 (0)
IBD+Ch (n = 16)	A	10	3 (1–4)	1.5 (1–3)	0 (0)
IBD+Ch (n = 16)	S	6	2.5 (1–4)	1 (1–2)	0 (0)
IBD+Ch+P (n = 8)	A	0	–	–	–
IBD+Ch+P (n = 8)	S	8	4 (1–4)	2 (1–3)	1.5 (1–4)
P (n = 1)	A	0	–	–	–
P (n = 1)	S	1	0 (0)	0 (0)	4 (4)
IBD+P (n = 2)	A	1	1 (1)	0 (0)	2 (2)
IBD+P (n = 2)	S	1	4 (4)	0 (0)	3 (3)
Total IBD (n = 39)	A	16	2 (1–4)	–	–
Total IBD (n = 39)	S	23	3 (1–4)	–	–
Total Ch (n = 30)	A	14	–	1.5 (1–3)	–
Total Ch (n = 30)	S	16	–	2 (1–3)	–
Total P (n = 11)	A	1	–	–	2 (2)
Total P (n = 11)	S	10	–	–	2.5 (1–4)

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A: cats without symptoms.

S: cats with symptoms.

Ctrl: controls.

IBD: cats with histopathological evidence of idiopathic inflammatory bowel disease.

Ch: cats with histopathological evidence of cholangitis.

IBD+Ch: cats with histopathological evidence of IBD and cholangitis.

IBD+Ch+P: cats with histopathological evidence of IBD, cholangitis, and pancreatitis.

P: cats with histopathological evidence of pancreatitis.

IBD+P: cats with histopathological evidence of IBD and pancreatitis.

Total IBD: all cats with histopathological evidence of IBD.

Total Ch: all cats with histopathological evidence of cholangitis.

Total P: all cats with histopathological findings of pancreatitis.

Histological severity scores for the different subgroups based on organs affected are shown in Table 3. There was a significant difference in histological severity of intestinal inflammation among the subgroups (χ ² = 34.084, df = 4, P < .001).

Table 3.

Medians and ranges (min–max) of histopathological severity scores of idiopathic inflammatory bowel disease (IBD), cholangitis, and pancreatitis in subgroups of the study population based on organ involvement

Groups	IBD Score	Cholangitis Score	Pancreatitis Score
Groups	Median (Range)	Median (Range)	Median (Range)
Ctrl (n = 8)	0^a (0)	0^a (0)	0^a (0)
IBD (n = 13)	2^b (1‐4)	0^a (0)	0^a (0)
Ch (n = 6)	0^a (0)	2^a (1–2)	0^a (0)
IBD+Ch (n = 16)	3^b,c (1–4)	1^a (1–3)	0^a (0)
IBD+Ch+P (n = 8)	4^c (1–4)	2^a (1–3)	1.5^a (1–4)
P (n = 1)	0 (0)	0 (0)	4 (4)
IBD+P (n = 2)	2.5 (1–4)	0 (0)	2.5 (2–3)

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a, b, c: in the same column of the table, medians followed by common letter do not differ significantly, according to the results of a series of Mann‐Whitney tests. Statistically significant differences exist between medians followed by different superscript letters.

Ctrl: controls

IBD: cats with histopathological evidence of idiopathic inflammatory bowel disease

Ch: cats with histopathological evidence of cholangitis

IBD+Ch: cats with histopathological evidence of IBD and cholangitis

IBD+Ch+P: cats with histopathological evidence of IBD, cholangitis, and pancreatitis

P: cats with histopathological evidence of pancreatitis

IBD+P: cats with histopathological evidence of IBD and pancreatitis

Histopathological Evidence of Cholangitis

Twenty‐five cats had gross abnormalities (albeit mild) during laparotomy, including a subtly mottled hepatic surface with pale (12) to light yellow discoloration (7) of the hepatic parenchyma and mild enlargement of the liver, evidenced by slightly rounded edges (16).

Histopathological evidence of cholangitis (Fig 1) was noted in 30 of the 47 ([63.8%]; 95% CI: 48.5–77.3%) cats of the study group; 16/27 ([59.3%]; 95% CI: 38.8–77.6%) of the symptomatic; and 14/20 ([70%] 95% CI: 45.7–88.1%) of the asymptomatic group. In 6 of them ([20%]; 95% CI: 10–30%) cholangitis was the only inflammatory lesion found, whereas in 24/30 ([80%]; 95% CI: 70–90%) cholangitis coexisted either with lesions of IBD (16/30 [53.3%]; 95% CI: 36.7–70%) or IBD and pancreatitis (8/30 [26.7%]; 95% CI: 16.7–36.7%). Specific lesions identified included inflammatory cell aggregates in portal areas, fibrosis, and hyperplasia of the bile ducts and oval cells. In the majority of cats (25/30 [83.3%]; 95% CI: 65.3–94.4%), the inflammatory cell component consisted primarily of lymphocytes and a relatively small number of plasma cells (lymphocytic cholangitis). Regarding the lymphocytic type of cholangitis, coexistence was observed in most of the cases (20/25 [80%]; 95% CI: 59.3–93.2%) either in combination with IBD (15/25 [60%]; 95% CI: 38.7–78.9%) or in the combination of triaditis (5/25 [20%]; 95% CI: 6.8–40.7%). In a small number of cases (5/30 [16.7%]; 95% CI: 5.6–34.7%), in addition to mononuclear cells a considerable population of neutrophils was present (chronic neutrophilic cholangitis).6 The majority of chronic neutrophilic cholangitis cases (4/5) also had IBD lesions (1/5) or triaditis (3/5). Cholestasis was evident in 18/30 (60%; 95% CI: 40.6–77.3%) cats with cholangitis. A small number of cats with cholangitis also had focal or multifocal hepatitis (4/30 [13.3%]; CI 95%: 3.8–30.7%). The variably sized focal hepatitis lesions typically had a mixed mononuclear cell and neutrophilic infiltrate with (3/30 [10%]; 95% CI: 2–27%) or without necrosis of the hepatic parenchyma.

The severity scores for cholangitis lesions for both the symptomatic and asymptomatic groups are shown in Table 2. Comparison of the severity scores for cholangitis between symptomatic and asymptomatic cats did not show a significant difference (P = .935).

A comparison of cholangitis severity scores among subgroups is shown in Table 3. Statistical analysis did not identify significant differences for the histological cholangitis scores between groups Ch and IBD+Ch (P = .685), Ch and IBD+Ch+P (P = .489), or IBD+Ch and IBD+Ch+P (P = .318).

Histopathological Evidence of Pancreatitis

Macroscopically, 10 cats had gross lesions in the pancreas at laparotomy, including edema (8), presence of nodules or plaques (7), firm consistency (1), hyperemia (5), necrosis (2), peripancreatic fat necrosis identified by the presence of saponified fat (2), or presence of a single pancreatic cyst (1).

Histopathological evidence of pancreatitis was noted in 12/47 (25.5%; 95% CI: 13.9–40.3%) cats of the study group; 11/27 (40.7%; 95% CI: 22.4–61.2%) in the symptomatic and 1/20 (5%; 95% CI: 0.1–24.9%) in the asymptomatic group. Pancreatitis lesions alone were observed in 1 cat, whereas the remaining 11/12 cats also showed concurrent lesions of IBD (3/12 cats) or IBD and cholangitis (8/12 cats).

Chronic lesions of pancreatitis characterized by mononuclear cell infiltration and interlobular fibrosis, intralobular fibrosis, or both were seen in 7 of the 12 pancreata with evidence of pancreatitis (Fig 2). Regarding this type of pancreatitis, coexistence was observed in all cases, either in combination with IBD (1/7) or in the combination of triaditis (6/7). In the remaining 5 of 12 cats with pancreatitis, the inflammatory cell component included a considerable number of neutrophils, apart from mononuclear cells and fibrosis. In 3 of these 5 cases, pancreatitis was classified as “chronic active”. In the remaining 2 cases, severe acute necrotic pancreatitis was present concurrently with chronic pancreatitis. In these cats extensive necrosis of the pancreatic parenchyma and peripancreatic fat was observed. The majority of pancreatitis cases with a neutrophilic cell component (4/5) also had IBD lesions (2/5) or triaditis (2/5).

The severity of pancreatitis lesions in symptomatic and asymptomatic cats of each group is shown in Table 2. Comparisons of severity scores between symptomatic and asymptomatic cats with lesions were not feasible because only 1 cat with pancreatitis was asymptomatic. However, a statistical comparison between symptomatic and asymptomatic cats considering cats without pancreatitis as having a score of 0 showed no statistically significant difference between symptomatic and asymptomatic cats (P = .08).

The scores of pancreatitis lesions of the subgroups based on organ involvement are presented in Table 3.

Hepatic Lipidosis

Microvesicular or macrovesicular lipidosis was observed in 13/47 (27.7%; 95% CI: 15.6–42.6%) cats of the study group, 10/27 (37%; 95% CI: 19.4–57.6%) symptomatic and 3/20 (15%; 95% CI: 3.2–37.9%) asymptomatic cats and in 2 of the 8 control cats. More specifically, 3 of the 13 IBD cats (23,1%), 2 of the 6 Ch cats (33.3%), 4 of the 16 IBD+Ch cats (25%), 3 of the 8 IBD+Ch+P cats (37.5%), and 1 of the 3 IBD+P cats (33.3%) showed evidence of hepatic lipidosis.

Signalment

Regarding the breed distribution within the 5 groups of cats, statistical analysis did not identify any statistically significant differences (z‐test P = .787). Because of the study design, the majority of cats enrolled were female, which precluded any further analysis of the sex distribution data. Regarding age, cats with histopathological evidence of cholangitis, pancreatitis, IBD, or a combination of these were significantly older compared with the control cats (median age, 2 years; in all comparisons P ≤ .04). Cats with triaditis (median age, 7.5 years) were significantly older than cats with lesions of IBD (median age, 3.5 years), cholangitis (median age, 3.5 years), or the combination of IBD and cholangitis (median age, 3.5 years; in all comparisons P ≤ .032). The median age of the cats as well as the differences among cat groups are presented in Table 4.

Table 4.

Medians and ranges (min–max) for age, results of CBC, and PTT for the control population and subgroups of the study population. Also shown is the number of cats with results outside the reference interval for each subgroup

Cats	Age (years)	HCT (%)	Νeutrophils/μL	Lymphocytes/μL	PTT (sec)
Group (N) (S/A)	Median (Range)	Median (Range) (n)	Median (Range) (n)	Median (Range) (n)	Median (Range) (n)
Ctrl (8) (0/8)	2^a (1–5)	35.4^a (30–42.3) (0)	4,810^a (3,151–10,550) (0)	3,951^a (1,576–6,955) (0)	92^a (78–118) (0)
IBD (13) (8/5)	3.5^b (1–16)	27.5^b (21.9–42.2) (4)	5233^a (2,194–20,604) (5)	2,093^b,c (330–8,337) (5)	92^a (68–111) (0)
Ch (6) (2/4)	3.5^b (3–10)	31.3^a,b (28.4–34.5) (0)	10,663^b (3,318–25,281) (2)	3,756^a,b (1,872–6,637) (0)	104^a,b (65–110) (0)
IBD+Ch (16) (6/10)	3.5^b (1–11)	31.3^a,b (19.3–39.9) (2)	9,267^b (943–17,620) (5)	1,904^c (597–4,304) (4)	84^a (72–401) (1)
IBD+Ch+P (8) (8/0)	7.5^c (3–14)	29.2^a,b (7.9–40.5) (3)	7,103^a,b (3,060–26,193) (3)	2,212^a,b,c (141–12,193) (5)	118^b (92–401) (3)
Reference interval	–	24–46	3,140–12,520	1,310–7,460	65–119
MSSDO_0.05	1.5	7.9	4,034	1,852	25.5
Kruskal‐Wallis P	.007	.062	.094	.021	.048

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MSSDO: minimum statistically significant difference observed at a significance level P = .05

Ctrl: controls.

IBD: cats with histopathological evidence of idiopathic inflammatory bowel disease.

Ch: cats with histopathological evidence of cholangitis.

IBD+Ch: cats with histopathological evidence of IBD and cholangitis.

IBD+Ch+P: cats with histopathological evidence of IBD, cholangitis, and pancreatitis.

S/A: symptomatic/asymptomatic cats in each subgroup.

HCT: hematocrit.

PTT: partial thromboplastine time.

History and Physical Examination

The presence or absence of clinical signs in all subgroups of cats is presented in Table 5. Comparisons of proportions of symptomatic vs. asymptomatic cats in each group with a series of z‐tests determined that cats of the IBD+Ch+P group were more likely to have clinical signs compared with the other subgroups (χ ² = 18.246, df = 4, P < .001; Table 5).

Table 5.

Comparisons between groups of cats relative to the presence (Yes) or absence (No) of (1) clinical signs in general (2) anorexia, polyphagia, or a normal appetite, and (3) vomiting

Groups	Clinical signs		Appetite			Vomiting
	Yes	No	Anorexia	Polyphagia	Normal Appetite	Yes	No
	n (%)	n (%)	n (%)	n (%)	n (%)	n (%)	n (%)
Ctrl (n = 8)	0 (0%)^a	8 (100%)	0 (0%)^a	0 (0%)^b	8 (100%)^a	0 (0%)^a	8 (100%)
IBD (n = 13)	8 (61.5%)^b	5 (38.5%)	6 (46.2%)^b,c	0 (0%)^b	7 (53.8%)^b	5 (38.5%)^b	8 (61.5%)
Ch (n = 6)	2 (33.3%)^a,b	4 (66.7%)	1 (16.7%)^a,b,c	1 (16.7%)^a,b	4 (66.7%)^a,b	2 (33.3%)^a,b	4 (66.7%)
IBD+Ch (n = 16)	6 (37.5%)^b	10 (62.5%)	3 (18.8%)^a,c	0 (0%)^b	13 (81.3%)^a,b	4 (25%)^a,b	12 (75%)
IBD+Ch+P (n = 8)	8 (100%)^c	0 (0%)	5 (62.5%)^b	3 (37.5%)^a	0 (0%)^c	7 (87.5%)^c	1 (12.5%)

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a, b, c: proportions in the same column followed by common superscript letter do not differ significantly based on the results of a series of z‐tests. By contrast, a statistically significant difference exists among proportions followed by different superscript letter.

Ctrl: controls.

IBD: cats with histopathological evidence of IBD.

Ch: cats with histopathological evidence of cholangitis.

IBD+Ch: cats with histopathological evidence of IBD and cholangitis.

IBD+Ch+P: cats with histopathological evidence of IBD, cholangitis, and pancreatitis.

The most common clinical signs reported in symptomatic cats were vomiting (20/27), lethargy (19/27), anorexia (18/27), weight loss (17/47), abnormal feces (from slightly soft feces to watery diarrhea; 15/27), poor body condition score (10/27), jaundice (10/47), and polyphagia (4/27). On physical examination, abnormal findings on palpation of the abdomen (e.g., mass effect suspicious of mesenteric lymph node enlargement; 4/47), thickened intestinal walls (4/47), mass effect on palpation of the anterior abdomen (4/47), pain (3/47), or hepatomegaly (2/47) also were common.

Comparisons of proportions of the cats presented with each clinical sign in each subgroup with a series of z‐tests determined that disruption of appetite (χ ² = 27,829, df = 8, P < .001) and vomiting (χ ² = 14.721, df = 4, P = .003) was present in a statistically significantly higher proportion of cats in the IBD+Ch+P group compared with any other groups. All 8 cats in the triaditis group were reported to have altered appetite, 3 of them presented with polyphagia along with poor body condition score and 5 had decreased appetite. Also, 7 of the 8 cats (87.5%) presented with vomiting (Table 5).

Clinical Pathology

A comparison of the results of the CBC among the 5 subgroups indicated statistically significant differences in hematocrit and number of circulating neutrophils and lymphocytes in the blood. The results of the comparisons, as well as the number of cats in each subgroup with values outside of the reference interval, are shown in Table 4. None of the cats in this study had an abnormal platelet count.

Common serum biochemistry findings were increased activities of ALT (11/47, 23.4%), ALP (11/47, 23.4%), and AST (2/47, 4.3%), and increased concentration of total bilirubin (14/47, 29.8%). Also, serum pancreatic markers fTLI (3/46, 6.5%) and fPLI (13/45, 28.9%) were increased in some cats. There were statistically significant differences among groups in ALT and AST activities, and P, ALB, and fPLI concentrations. The results of these comparisons as well as number of cats in each group with values outside of the reference interval are shown in Table 6. In addition, serum fPLI results in cats with and without histopathologic evidence of pancreatitis are shown in Table 7.

Table 6.

Medians and ranges (min–max) for various serum biochemical parameters for the control population and subgroups of the study population. Also shown is the number of cats with results outside the reference interval for each subgroup

Cats	ALT (U/L)	AST (U/L)	P (mg/dL)	ALB (g/dL)	fPLI (μg/L)
Group (N) (S/A)	Median (Range) (n)	Median (Range) (n)	Median (Range) (n)	Median (Range) (n)	Median (Range) (n)
Ctrl (8) (0/8)	35.5^a (25–58) (0)	21.5^a (15–32) (0)	5.7^a (5.1–6.7) (0)	3.9^a (3.2–3.9) (0)	1.5^a (1.3–1.8) (0)
IBD (13) (8/5)	47.0^a,b (24–283) (3)	26.0^a,b (16–174) (1)	5.5^a,b (3.3–7.5) (2)	3.7^a,b (2.6–4.5) (1)	1.7^a,c (1.2–45) (2)
Ch (6) (2/4)	39.0^a (23–180) (1)	25.5^a,b (20–135) (1)	5.0^b (4.5–6) (0)	3.4^b (3.2–3.8) (0)	4.2^b (1.6–6.6) (4)
IBD+Ch (16) (6/10)	32.0^a (14–710) (4)	33.0^a,b (15–70) (0)	5.0^b (4–8) (1)	3.5^b (2.8–3.9) (1)	1.9^a,c (1.2–18.5) (3)
IBD+Ch+P (8) (8/0)	68.0^b (48–210) (3)	52.0^b (31–87) (0)	4.2^c (3.4–6.4) (1)	3.5^b (2.3–4.4) (3)	2.0^c (1.5–4.0) (1)
Reference interval	21–103	15–97	3.5–6.7	3–4.8	0–3.5
MSSDO_0.05	29.0	30.5	0.7	0.4	0.5
Kruskal‐Wallis P	.026	.068	.064	.098	.022

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MSSDO: minimum statistically significant difference observed at a significance level P = .05

Ctrl: controls

IBD: cats with histopathological evidence of idiopathic inflammatory bowel disease

Ch: cats with histopathological evidence of cholangitis

IBD+Ch: cats with histopathological evidence of IBD and cholangitis

IBD+Ch+P: cats with histopathological evidence of IBD, cholangitis, and pancreatitis

S/A: symptomatic/asymptomatic cats in each subgroup

ALT: alanine transaminase

AST: aspartate transaminase

P: phosphorus

ALB: albumin

fPLI: feline pancreatic lipase immunoreactivity concentration (as measured by Spec fPL^®).

Table 7.

Numbers of cats with serum fPLI concentrations above or below the cut‐off value of 3.5 μg/L

fPLI		Histopathological results
fPLI		Cats without any inflammatory lesions (N = 8)	Cats with other inflammatory lesions, but no lesions of pancreatitis (N = 34)	Cats with pancreatitis lesions with or without other inflammatory lesions (N = 11)
Cut‐off value (μg/L)	Result (number of cats)	Cats without any inflammatory lesions (N = 8)
3.5	Negative (n ≤ 3.5)	8	25	7
3.5	Positive (n > 3.5)	0	9	4

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Diagnostic Imaging

Diagnostic imaging findings were normal in all cats of the control group and in many cats of the study group. All 47 cats of the study group had thoracic radiographs taken and 38 also had abdominal radiographs taken. No abnormalities were detected on any of the thoracic radiographs. On abdominal radiographs, 31/38 (81.6%) cats had normal findings, whereas radiography disclosed hepatomegaly in 3/38 (7.9%) cats and an abnormal air distribution in the intestinal lumen indicative of enteritis in 4/38 (10.5%) cats. Of the 45 cats of the study group that underwent abdominal ultrasound examination, 26 (57.8%) did not have any abnormalities. In the remaining 19 cats, ultrasonography of the abdomen disclosed the presence of various abnormalities: mesenteric lymph node enlargement (9/45, 20%), increased echogenicity of the feline liver (7/45, 15.6%), bowel dysmotility indicative of ileus (2/45, 4.4%), thickening of the intestinal wall (2/45, 4.4%), hepatomegaly (2/45, 4.4%), hepatic cyst formation (1/45, 2.2%), enlarged and hypoechogenic pancreas (1/45, 2.2%), decreased echogenicity of the pancreas with cyst formation (1/45, 2.2%), increased echogenicity in the anterior abdomen (1/45, 2.2%), or peritoneal effusion (1/45, 2.2%).

Correlations between Histopathological Scores and Clinicopathological Parameters

A mild, positive correlation was observed between the histopathological IBD score and the number of comorbidities in addition to IBD (rho = +0.367, P = .022, n = 39). Cats without histopathologic evidence of IBD were excluded from this correlation analysis.

The finding of a negative correlation was detected between the score of IBD lesions and the results of PCV (rho = −0.336, P = .013), blood lymphocyte count (rho = −0.413, P = .002), and concentration of ALB (rho = −0.310, P = .022).

A mild positive correlation was detected between the histopathologic cholangitis score and blood neutrophil count (rho = +0.323, P = .018) or fPLI (rho = +0.307, P = .025), whereas the correlation with the concentration of ALB (rho = −0.332, P = .014) was negative.

A mild positive correlation was detected between pancreatitis score and age (rho = +0.400, P = .03), PTT (rho = +0.433, P = .002), ALT activity (rho = +0.269, P = .049), and concentrations of LIPA (rho = +0.287, P = .039) and fPLI (rho = +0.287, P = .037). Negative correlation was identified between the histopathologic pancreatitis score and PVC (rho = −0.271, P = .048).

A strong positive correlation was observed between the hepatic lipidosis score and activities of ALP (rho = +0.610, P < .001), AST (rho = +0.487, P < .001), γGT (rho = +0.467, P < .001), or concentration of TBIL (rho = +0.474, P < .001). A mild positive correlation was observed between ALT activity (rho = +0.388, P = .003) and total serum lipase activity (rho = +0.288, P = .037).

Discussion

To our knowledge, ours is the first prospective clinical study to investigate coexisting feline inflammatory liver, pancreatic, and small bowel disease in clinical cases. Our study indicated that histopathologic lesions of enteritis, cholangitis, pancreatitis, or some combination of these might coexist in cats, presenting a complex and in some cases severe inflammatory disease. Histopathological evidence of inflammation in 1, 2, or all 3 organs was present in 27/39 (69.2%; 95% CI: 56.4–79.5%) of symptomatic cats. Triaditis was present in 8/27 ([29.6%]; 95% CI: 14.8–48.1%) cases and the combination of IBD with either cholangitis (6/27) or pancreatitis (2/27) in 8/27 ([29.6%]; 95% CI: 14.8–48.1%) cats. Furthermore, our study identified evidence of inflammation in a high proportion of asymptomatic cats. Histopathological lesions of either cholangitis or enteritis alone, or combinations of inflammatory infiltration in 2 of the 3 organs were identified in the absence of clinical signs in 20/39 ([51.3%]; 95% CI: 38.5–64.1%) cats. The combination of IBD and cholangitis (10/20) or pancreatitis (1 case) was present in 11/20 ([55%]; 95% CI: 35–75%) asymptomatic cats. These findings suggest that IBD and combinations of inflammatory diseases of 2 organs begin before the onset of clinical signs or that in some cases clinical signs are so mild and transient that are missed by the owner. Furthermore, in our study pancreatitis alone or triaditis was only present in cats that were symptomatic. Also, the combination of cholangitis and pancreatitis without concurrent lesions of IBD was not detected. In addition, the histological severity of IBD lesions was positively correlated with the number of comorbidities. Thus, these results suggest that the severity of IBD lesions and the presence of pancreatitis play critical roles in the overt clinical disease, so‐called triaditis.

As previously mentioned, current knowledge related to triaditis in cats is mainly derived from a small number of retrospective studies based on necropsy findings, and differences in the histopathological classification of the 3 component diseases make comparisons among different studies difficult.2 In 1 study on cholangiohepatitis in cats, concurrent pancreatitis and IBD lesions were reported in 7/18 (38.9%) cholangiohepatitis cases.29 In a similar, more recent study, triaditis was identified in 10/31 (32.3%) cases with cholangitis.31 The concurrent presence of pancreatitis and IBD was reported in 7/14 (50%) cats with various histological subtypes of inflammatory liver disease.1, 2 In our study, triaditis was identified in 8/30 ([26.7%]; 95% CI: 16.7–36.7%) cats with cholangitis. Regarding the type of cholangitis, triaditis was detected in 5/25 (20%; 95% CI: 6.8–40.7%) cases of lymphocytic cholangitis and in (3/5) cats with chronic neutrophilic cholangitis. In the previous studies, cats that had either been euthanized or had died were enrolled, which likely indicates more severe disease in these patients.

In our study, histopathological evaluation of biopsy specimens from the feline liver, pancreas, and small intestine was performed. Although highly localized inflammation can be missed with this technique, it remains the ‘gold standard’ for diagnosing cholangitis,39, 40, 41 pancreatitis,14, 35, 42 or IBD.4, 5 Although invasive, open surgery was selected instead of other methods because laparotomy allows direct visual inspection of the organs and detection of gross lesions, and also facilitates control of hemorrhage during liver biopsy. Laparotomy also allows for the collection of full‐thickness tissue samples from all segments of the small intestine, which facilitates the differentiation of IBD from lymphoma.43

In this study, lymphocytes and plasma cells were the predominant cell types seen in all sites evaluated. In cats, chronic inflammatory liver disease mostly affects the biliary tree and portal areas and less commonly the hepatic parenchyma.6, 44, 45 Also, cholangitis is predominantly lymphocytic.40, 41 According to a recent study, many cases of lymphocytic cholangitis remain subclinical, whereas concurrent inflammatory diseases such as pancreatitis, IBD, or both more frequently might be associated with clinical signs.31 Regarding pancreatic inflammation, cats with chronic pancreatitis were likely to have concurrent disease.30 Finally, lymphoplasmocytic enteritis is considered the most common type of IBD in the cat.36, 46, 47

In our study, no statistically significant differences in the severity of histopathological lesions of either a single organ or a combination of several organs were identified between symptomatic and asymptomatic cats. However, epithelial dysplasia was not seen in asymptomatic cats with lesions suggesting IBD. Also, the severity of cholangitis lesions, when present alone, did not differ significantly from when present along with IBD or both IBD and pancreatitis lesions. In contrast, the histopathological lesions of IBD in cats with triaditis were significantly more severe than in cats where IBD lesions were present alone. These findings support the hypothesis that in this multifactorial syndrome, IBD might play a predominant role.

Regarding age predisposition for the 3 component diseases of triaditis, lymphocytic cholangitis has been described to affect mostly older cats,23 whereas other investigators also have reported the disease in young cats.41, 48, 49, 50 Although pancreatitis potentially affects cats of all ages, lymphocytic pancreatitis has been reported to occur more commonly in older cats.1, 30, 37 Inflammatory bowel disease is reported to be more common in middle‐aged (5–8 years old) and older (>8 years old) cats.36, 47, 51 However, the disease might occur at any age, including in cats <1 year of age.52 Our study indicated that cats with inflammatory lesions were significantly older compared with controls (median age, 2 years), but this observation might have been caused by the fact that control cats were selected from cats presented for ovariohysterectomy. Furthermore, it was shown that the severity of pancreatitis was positively related with age and that cats with triaditis (median age, 7.5 years) were significantly older than cats with lesions of cholangitis, IBD, or IBD in combination with cholangitis (median age, 3.5 years). Although a statistically significant correlation between age and the number of comorbidities with IBD could not be confirmed, an increased frequency of older cats among cats with triaditis was noted, compared to cats with only 2 comorbidities or IBD only.

Regarding the clinical presentation of cats with a combination of inflammatory lesions in several organs, various nonspecific clinical signs that are common to all 3 disease components were reported by owners. Their incidence was increased in cats with triaditis in which altered appetite (either increased or decreased) or vomiting was the predominant clinical signs.

Hematological and serum biochemical findings in cats enrolled in our study were as expected for the diseases identified.39, 53, 54, 55, 56 Depending on the severity of the inflammatory lesions, IBD was associated with decreases in PCV, peripheral lymphocyte counts, and serum albumin concentration. Cholangitis was associated with a decrease in ALB and an increase in the numbers of blood neutrophils, as well as an increase in serum fPLI. Furthermore, pancreatitis was associated with an increase in PTT, ALT activity, total lipase activity, fPLI, and a decrease in PCV.

As shown by our data, hepatic lipidosis often is associated with cholangitis, pancreatitis, IBD,12, 57, 58 or some combination of these and was found in 13/47 (27.7%; 95% CI: 15.6–42.6%) cats in our study. The presence of hepatic lipidosis can affect hepatic function and consequently the clinical and laboratory variables of the disease. In our study, severe lipidosis was strongly associated with increased serum activities of ALP, AST, and γGT and increased concentrations of TBIL, and mildly associated with increased serum activity of ALT or serum total lipase activity.

Minimally invasive, reliable, and specific tests for the diagnosis of pancreatitis are greatly needed. Although histopathologic examination of pancreatic biopsy specimens allows for definitive diagnosis when inflammatory infiltrates are observed, pancreatic biopsy is not always feasible. Furthermore, focal inflammatory lesions of pancreatitis might escape diagnosis.37, 59 Serum total lipase activity60, 61, 62 and serum fTLI63, 64, 65, 66 are not considered sensitive or specific for the diagnosis of exocrine pancreatic disease in cats, and this was confirmed in our study for the fTLI test. Concerning serum total lipase activity, although a mild positive correlation was detected between median activity of this test and the severity score of pancreatitis, the difference between the controls and the triaditis group was not statistically significant. Feline pancreatic lipase immunoreactivity (fPLI, as measured by Spec fPL^®) is an assay that specifically quantifies lipase that originates from pancreatic acinar cells.32, 34 The fPLI's overall sensitivity for the diagnosis of pancreatitis has been estimated to be 67% (ranging from 54% in mild cases to 100% in moderate‐to‐severe cases of pancreatitis) and its specificity has reached 100% in healthy cats and 91% in cats with gastrointestinal disease without histopathological lesions in the pancreas.62, 64, 65, 66 In a recent study of naturally occurring pancreatitis, the sensitivity of the method was reported to be 78%.¹³ As shown in our study, median fPLI in cats with triaditis was significantly higher when compared with the control group, although the median score of pancreatitis recorded in the triaditis group was suggestive of mild‐to‐moderate pancreatitis and the median fPLI did not exceed the upper limit of the reference interval (3.5 μg/L). Moreover, a statistically significant increase in the median value of the variable also was observed in group Ch, compared with controls and all of the other groups of cats. One possible explanation is that a number of cats from group Ch might have had localized pancreatitis that was missed on histopathology (because only a single biopsy specimen was evaluated for the purpose of our study). Consequently, additional studies involving larger numbers of cats with triaditis and of variable severity pancreatitis are needed. Several pancreatic biopsy specimens should be collected and evaluated, and serum fPLI must be considered along with other clinical and laboratory findings to maximize the diagnostic capabilities for pancreatitis and triaditis.

The liver, pancreas, and intestine are closely associated anatomically and functionally. Both the gut and the liver play important roles in immunology. The complex ecosystem of the intestinal microbiota influences numerous functions, and overall health and any disruptions of the interaction with the local immune system can lead to gastrointestinal disease.67, 68, 69, 70, 71, 72 Current studies raise the possibility that enteric bacteria are related to immune‐mediated cholangitis and pancreatitis.73, 74, 75 Overall, an autoimmune process might be the underlying cause of triaditis in cats.2, 41

Our study has some limitations that deserve mention. The examination of a relatively small population of symptomatic cats might have limited the power of this study to detect differences among groups. Only 1 tissue specimen per site was collected from the client‐owned cats because of the inherent risk of biopsy, especially for critically ill cats, and this might have limited the accuracy of histopathological assessment. In addition, only 1 pathologist examined the histological sections. However, this avoided the problem of suboptimal interobserver agreement.

In conclusion, this prospective study evaluated the prevalence of coexistence of IBD, cholangitis, and pancreatitis in both symptomatic and asymptomatic cats. No statistically significant differences in the severity of histopathological lesions of either a single organ or a combination of several organs were identified between symptomatic and asymptomatic cats. The histopathological lesions of IBD in cats with triaditis were significantly more severe than in cats where IBD lesions were present alone, suggesting that IBD might play a role in the development of triaditis. Further research is essential to better understand the complex interactions among inflammation in these organs and thus improve our diagnostic and therapeutic capabilities.

Supporting information

Data S1. Supplementary information regarding biopsies from the liver, pancreas, and intestine.

Data S2. Supplementary information regarding symptomatic cats that were excluded from the study.

Data S3. Supplementary information regarding asymptomatic cats that were excluded from the study.

Click here for additional data file.^{(12.5KB, pdf)}

Acknowledgments

The authors acknowledge the contribution of G.C. Menexes, Laboratory of Agronomy, School of Agriculture, Aristotle University of Thessaloniki, Greece, for performing statistical analysis. The authors thank all cat owners, veterinarians, students, and clinic staff members who participated in the study.

The first author (F.F.) was supported by the NSF‐national scholarships foundation (grant code number 5321).

Conflict of Interest Declaration: Authors declare no conflicts of interest.

Off‐label Antimicrobial Declaration: Authors declare no off‐label use of antimicrobials.

The study was conducted at the Companion Animal Clinic, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Greece.

The data were presented in part at the 22nd Annual Congress of the European College of Veterinary Internal Medicine‐Companion Animals, 6–8 September 2012, Maastricht, The Netherlands

Footnotes

ADVIA® 120 Hematology System, Siemens Medical Solutions, Fernwald, Germany

Clinical Chemistry Analyser, Flexor Ε, Vital Scientific N.V, Dieren, The Netherlands

Roche 9180, Electrolyte Analyzer, Roche Diagnostics, Mannheim, Germany

⁴

Veterinary Coagulation Analyser, SCA 2000®, Synbiotics®, San Diego, California

⁵

Immulite 1000, Cirrus Diagnostics Inc., Los Angeles

⁶

Spec fPL, Idexx Laboratories, Westbrook, Maine

⁷

Gastrointestinal Laboratory, Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, Texas A&M University

⁸

Feline Leukemia Virus Antigen/Feline Immunodeficiency Virus Antibody Test Kit, SNAP Combo Plus, IDDEX Laboratories, Inc. Westbrook

⁹

ImmunoComb®, Feline Corona Virus Antibody Test Kit, Biogal Galed Laboratories, Kibbutz Galed, Israel

¹⁰

X‐ray generator, Polydoros 80, Siemens, Munich, Germany

¹¹

Ultrasound system Apogee 800, Advanced Technologies Laboratory, Ambler, Pennsylvania

¹²

Statistical pack IBM SPSS v.20.0, Chicago: Illinois

¹³

Forman MA, Shiroma J, Armstrong PJ, et al. Evaluation of feline pancreas‐specific lipase (Spec fPL) for the diagnosis of feline pancreatitis (Abstract). J Vet Intern Med 2009; 23:733–734

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Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

Data S1. Supplementary information regarding biopsies from the liver, pancreas, and intestine.

Data S2. Supplementary information regarding symptomatic cats that were excluded from the study.

Data S3. Supplementary information regarding asymptomatic cats that were excluded from the study.

Click here for additional data file.^{(12.5KB, pdf)}

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[jvim14356-bib-0054] 54. Hall EJ, German AJ. Diseases of the Small Intestine. Gastrointestinal Disease In: Ettinger SJ, Feldman EC, eds. Textbook of Veterinary Internal Medicine: Diseases of the Dog and Cat, 7th ed Canada: Saunders, Elsevier; 2010;1479–1608. [Google Scholar]

[jvim14356-bib-0055] 55. Hitt EM. Inflammatory Liver Diseases In: August JR, ed. Consultations in Feline Internal Medicine, 6th ed St Louis: Saunders/Elsevier; 2010:213–224. [Google Scholar]

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PERMALINK

Prevalence and Clinicopathological Features of Triaditis in a Prospective Case Series of Symptomatic and Asymptomatic Cats

FC Fragkou

KK Adamama‐Moraitou

T Poutahidis

NN Prassinos

M Kritsepi‐Konstantinou

PG Xenoulis

JM Steiner

JA Lidbury

JS Suchodolski

TS Rallis

Abstract

Background

Hypothesis/Objectives

Animals

Methods

Results

Conclusions and Clinical Importance

Abbreviations

Materials and Methods

Study Design

Symptomatic Cats

Asymptomatic Cats

Clinical Examination and Diagnostic Testing

Histopathology

Figure 1.

Figure 2.

Figure 3.

Statistical Analyses

Results

Histopathological Examination

Symptomatic cats

Asymptomatic Cats

Assignment of Cats to Groups

Histopathological Evidence of IBD

Table 1.

Table 2.

Table 3.

Histopathological Evidence of Cholangitis

Histopathological Evidence of Pancreatitis

Hepatic Lipidosis

Signalment

Table 4.

History and Physical Examination

Table 5.

Clinical Pathology

Table 6.

Table 7.

Diagnostic Imaging

Correlations between Histopathological Scores and Clinicopathological Parameters

Discussion

Supporting information

Acknowledgments

Footnotes

References

Associated Data

Supplementary Materials

ACTIONS

PERMALINK

RESOURCES

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