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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1991 Feb 1;88(3):1004–1008. doi: 10.1073/pnas.88.3.1004

Specific initiation by RNA polymerase I in a whole-cell extract from yeast.

M C Schultz 1, S Y Choe 1, R H Reeder 1
PMCID: PMC50943  PMID: 1992452

Abstract

A protocol is described for making a soluble whole-cell extract from yeast (Saccharomyces cerevisiae) that supports active and specific transcription initiation by RNA polymerases I, II, and III. Specific initiation by polymerase I decreases in high-density cultures, paralleling the decrease in abundance of the endogenous 35S rRNA precursor. This extract should be useful for studying the molecular mechanisms that regulate rRNA transcription in yeast.

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Selected References

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