Skip to main content
. 2016 May 18;7(24):37000–37012. doi: 10.18632/oncotarget.9454

Figure 6. Knockdown of GLRX3 in NPC cells decreases pAKT independent of reactive oxygen species (ROS) generation.

Figure 6

(A) Western blot analysis of protein levels of pAkt and Akt in shGLRX3-HONE1/CNE2 cell lines. (B) ROS were detected by DCFH-DA staining with green fluorescent signal (magnification ×200). (C) The intensity of ROS was calculated as relative light unit. Data are mean±SD from three experiments (D–E) ROS detection in shGLRX3-HONE1 and shGLRX3-CNE2 cells treated with and without ROS inhibitor 5 mM NAC for 48 h, followed by intensity analysis. (F) Western blot analysis of pAkt protein level. Data are mean ± SD from three experiments. (**p < 0.01)