Figure 3. ZC3H15 knockdown inhibited HCC cell growth in vitro and in vivo.

A. ZC3H15 protein levels were examined by western blotting in HCC and other cell lines. B, C. ZC3H15 was knocked down in SMMC-7721 cells by lentivirus delivered shRNA targeting ZC3H15. The effects of the three shRNA were examined by qPCR and western blotting assays. D, E. Proliferation of siZC3H15-SMMC-7721 and control cells was detected by quantifying EGFP fluorescence intensity. F. A panel clonogenic assay of two HCC cell lines after ZC3H15 knockdown. The HCC cells (2×10³ cells per well) were seeded in 6-well plates and incubated for 10 days, and then the number of cell colonies was counted. G. SMMC7721-control and siZC3H15 cells (5×10⁶ cells per mouse) were inoculated into flanks of nude mice and tumor growth was observed for 3 weeks. Error bars represent the SEM *P<0.05, **P<0.01.