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Screening reactions used to generate mutability landscapes for enzymatic activity. A) The BSLA‐catalyzed hydrolysis of p‐nitrophenyl butyrate (1), yielding p‐nitrophenol (2) and butyric acid (3). B) The 4‐OT‐catalyzed Michael‐type addition of butanal (4) to trans‐β‐nitrostyrene (5), yielding chiral γ‐nitroaldehyde 6. C) The Bgl3‐catalyzed glycoside‐bond cleavage of fluorescein di‐(β‐d‐glucopyranoside) (7), yielding fluorescein (8) and β‐d‐glucopyranose (9).
