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A) FLIM of BODIPY‐C10/PD DOPC GUVs during irradiation. Single GUV, which is shown by the red arrow, was irradiated at 453 nm by zooming in 5 times, for periods of time shown in each image. B) Fluorescence lifetime histograms of BODIPY‐C10 in the irradiated vesicle obtained upon 480 nm excitation. The lifetime increases from 1509±26 to 2254±53 ps corresponds to viscosity increase from 170±5 to 332±14 cP.
