Figure 6. Evaluation of 6V-VST by IFNγ ELISpot and Gamma capture.
A) ELISpot evaluation of 6V-VST (n=7) showed activity against included HPIV3 pepmixes (Fus, NP) in seven of nine 6V-VST products. B) 6V-VSTs recognized multiple epitopes and C) multiple viruses per product as determined by ELISpot evaluation. D) The majority of 6V-VST recognized at least one epitope from multiple viruses. E) 6V-VSTs isolated IFNγ capture following stimulation with HPIV3 pepmixes were predominantly CD45RA-CCR7-, but differed with regard to CD62L expression, with CD4+ T-cells expressing CD62L in two of three products tested, while CD8+ T-cells were uniformly CD62L-.