Figure 5.

Effect of CSF2 and sex on cleavage and development of embryos to the blastocyst stage. The top panel shows representative images of a female (A) and a male (B) Day 7 blastocyst labeled with Hoechst (all nuclei) and anti-CDX2 (trophectoderm (TE) cells). The graphs represent least-squares means ± s.e.m. for cleavage (C), percent putative zygotes developing to the blastocyst stage (D), numbers of total (E), inner cell mass (ICM; F), trophectoderm (TE; G), and the ratio of TE to ICM cell numbers (H). The experiment was replicated nine times with a total of 351–427 putative zygotes per treatment group. P value for the main effects of sex, CSF2 treatment (trt) and the interaction are shown for all effects where P < 0.10 or less. In addition, pairwise comparisons of effects of CSF2 were performed separately for female and male embryos and those effects where P was <0.10 or less are indicated by the brackets.