Abstract
The acute inflammatory response requires that circulating leukocytes bind to and penetrate the vascular wall to access the site of injury. Several receptors have been implicated in this interaction, including a family of putative carbohydrate-binding proteins. We report here the identification of an endogenous carbohydrate ligand for one of these receptors, endothelial-leukocyte adhesion molecule 1 (ELAM-1). Radiolabeled COS cells transfected with a plasmid containing the cDNA for ELAM-1 were used as probes to screen glycolipids extracted from human leukocytes. COS cells transfected with this plasmid adhered to a subset of sialylated glycolipids resolved on TLC plates or adsorbed on polyvinyl chloride microtiter wells. Adhesion to these glycolipids required calcium but was not inhibited by heparin, chondroitin sulfate, keratan sulfate, or yeast phosphomannan. Monosaccharide composition, linkage analysis, and fast atom bombardment mass spectrometry of the glycolipids indicate that the ligands for ELAM-1 are terminally sialylated lactosylceramides with a variable number of N-acetyllactosamine repeats and at least one fucosylated N-acetylglucosamine residue.
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Selected References
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- Aruffo A., Seed B. Molecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system. Proc Natl Acad Sci U S A. 1987 Dec;84(23):8573–8577. doi: 10.1073/pnas.84.23.8573. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bevilacqua M. P., Pober J. S., Mendrick D. L., Cotran R. S., Gimbrone M. A., Jr Identification of an inducible endothelial-leukocyte adhesion molecule. Proc Natl Acad Sci U S A. 1987 Dec;84(24):9238–9242. doi: 10.1073/pnas.84.24.9238. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bevilacqua M. P., Stengelin S., Gimbrone M. A., Jr, Seed B. Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins. Science. 1989 Mar 3;243(4895):1160–1165. doi: 10.1126/science.2466335. [DOI] [PubMed] [Google Scholar]
- Blackburn C. C., Swank-Hill P., Schnaar R. L. Gangliosides support neural retina cell adhesion. J Biol Chem. 1986 Feb 25;261(6):2873–2881. [PubMed] [Google Scholar]
- Brandley B. K., Schnaar R. L. Cell-surface carbohydrates in cell recognition and response. J Leukoc Biol. 1986 Jul;40(1):97–111. doi: 10.1002/jlb.40.1.97. [DOI] [PubMed] [Google Scholar]
- Brandley B. K., Swiedler S. J., Robbins P. W. Carbohydrate ligands of the LEC cell adhesion molecules. Cell. 1990 Nov 30;63(5):861–863. doi: 10.1016/0092-8674(90)90487-y. [DOI] [PubMed] [Google Scholar]
- Camerini D., James S. P., Stamenkovic I., Seed B. Leu-8/TQ1 is the human equivalent of the Mel-14 lymph node homing receptor. Nature. 1989 Nov 2;342(6245):78–82. doi: 10.1038/342078a0. [DOI] [PubMed] [Google Scholar]
- Constantine-Paton M., Blum A. S., Mendez-Otero R., Barnstable C. J. A cell surface molecule distributed in a dorsoventral gradient in the perinatal rat retina. Nature. 1986 Dec 4;324(6096):459–462. doi: 10.1038/324459a0. [DOI] [PubMed] [Google Scholar]
- Dahms N. M., Schnaar R. L. Ganglioside composition is regulated during differentiation in the neuroblastoma X glioma hybrid cell line NG108-15. J Neurosci. 1983 Apr;3(4):806–817. doi: 10.1523/JNEUROSCI.03-04-00806.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dodd J., Jessell T. M. Lactoseries carbohydrates specify subsets of dorsal root ganglion neurons projecting to the superficial dorsal horn of rat spinal cord. J Neurosci. 1985 Dec;5(12):3278–3294. doi: 10.1523/JNEUROSCI.05-12-03278.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fukuda M. N., Dell A., Tiller P. R., Varki A., Klock J. C., Fukuda M. Structure of a novel sialylated fucosyl lacto-N-norhexaosylceramide isolated from chronic myelogenous leukemia cells. J Biol Chem. 1986 Feb 15;261(5):2376–2383. [PubMed] [Google Scholar]
- Fukuda M., Bothner B., Ramsamooj P., Dell A., Tiller P. R., Varki A., Klock J. C. Structures of sialylated fucosyl polylactosaminoglycans isolated from chronic myelogenous leukemia cells. J Biol Chem. 1985 Oct 25;260(24):12957–12967. [PubMed] [Google Scholar]
- Geng J. G., Bevilacqua M. P., Moore K. L., McIntyre T. M., Prescott S. M., Kim J. M., Bliss G. A., Zimmerman G. A., McEver R. P. Rapid neutrophil adhesion to activated endothelium mediated by GMP-140. Nature. 1990 Feb 22;343(6260):757–760. doi: 10.1038/343757a0. [DOI] [PubMed] [Google Scholar]
- Geoffroy J. S., Rosen S. D. Demonstration that a lectin-like receptor (gp90MEL) directly mediates adhesion of lymphocytes to high endothelial venules of lymph nodes. J Cell Biol. 1989 Nov;109(5):2463–2469. doi: 10.1083/jcb.109.5.2463. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goelz S. E., Hession C., Goff D., Griffiths B., Tizard R., Newman B., Chi-Rosso G., Lobb R. ELFT: a gene that directs the expression of an ELAM-1 ligand. Cell. 1990 Dec 21;63(6):1349–1356. doi: 10.1016/0092-8674(90)90430-m. [DOI] [PubMed] [Google Scholar]
- Hakomori S., Nudelman E., Levery S. B., Patterson C. M. Human cancer-associated gangliosides defined by a monoclonal antibody (IB9) directed to sialosyl alpha 2 leads to 6 galactosyl residue: a preliminary note. Biochem Biophys Res Commun. 1983 Jun 29;113(3):791–798. doi: 10.1016/0006-291x(83)91069-0. [DOI] [PubMed] [Google Scholar]
- Hardy M. R., Townsend R. R., Lee Y. C. Monosaccharide analysis of glycoconjugates by anion exchange chromatography with pulsed amperometric detection. Anal Biochem. 1988 Apr;170(1):54–62. doi: 10.1016/0003-2697(88)90089-9. [DOI] [PubMed] [Google Scholar]
- Hession C., Osborn L., Goff D., Chi-Rosso G., Vassallo C., Pasek M., Pittack C., Tizard R., Goelz S., McCarthy K. Endothelial leukocyte adhesion molecule 1: direct expression cloning and functional interactions. Proc Natl Acad Sci U S A. 1990 Mar;87(5):1673–1677. doi: 10.1073/pnas.87.5.1673. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Johnston G. I., Cook R. G., McEver R. P. Cloning of GMP-140, a granule membrane protein of platelets and endothelium: sequence similarity to proteins involved in cell adhesion and inflammation. Cell. 1989 Mar 24;56(6):1033–1044. doi: 10.1016/0092-8674(89)90636-3. [DOI] [PubMed] [Google Scholar]
- Lasky L. A., Singer M. S., Yednock T. A., Dowbenko D., Fennie C., Rodriguez H., Nguyen T., Stachel S., Rosen S. D. Cloning of a lymphocyte homing receptor reveals a lectin domain. Cell. 1989 Mar 24;56(6):1045–1055. doi: 10.1016/0092-8674(89)90637-5. [DOI] [PubMed] [Google Scholar]
- Lowe J. B., Stoolman L. M., Nair R. P., Larsen R. D., Berhend T. L., Marks R. M. ELAM-1--dependent cell adhesion to vascular endothelium determined by a transfected human fucosyltransferase cDNA. Cell. 1990 Nov 2;63(3):475–484. doi: 10.1016/0092-8674(90)90444-j. [DOI] [PubMed] [Google Scholar]
- Macher B. A., Beckstead J. H. Distribution of VIM-2 and SSEA-1 glycoconjugate epitopes among human leukocytes and leukemia cells. Leuk Res. 1990;14(2):119–130. doi: 10.1016/0145-2126(90)90040-g. [DOI] [PubMed] [Google Scholar]
- Macher B. A., Buehler J., Scudder P., Knapp W., Feizi T. A novel carbohydrate, differentiation antigen on fucogangliosides of human myeloid cells recognized by monoclonal antibody VIM-2. J Biol Chem. 1988 Jul 25;263(21):10186–10191. [PubMed] [Google Scholar]
- Phillips M. L., Nudelman E., Gaeta F. C., Perez M., Singhal A. K., Hakomori S., Paulson J. C. ELAM-1 mediates cell adhesion by recognition of a carbohydrate ligand, sialyl-Lex. Science. 1990 Nov 23;250(4984):1130–1132. doi: 10.1126/science.1701274. [DOI] [PubMed] [Google Scholar]
- Polte T., Newman W., Gopal T. V. cDNA for endothelial leukocyte adhesion molecule 1 (ELAM1): sequence differences. Nucleic Acids Res. 1990 Feb 25;18(4):1083–1083. doi: 10.1093/nar/18.4.1083. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sharon N., Lis H. Lectins as cell recognition molecules. Science. 1989 Oct 13;246(4927):227–234. doi: 10.1126/science.2552581. [DOI] [PubMed] [Google Scholar]
- Springer T. A. Adhesion receptors of the immune system. Nature. 1990 Aug 2;346(6283):425–434. doi: 10.1038/346425a0. [DOI] [PubMed] [Google Scholar]
- Svennerholm L., Fredman P. A procedure for the quantitative isolation of brain gangliosides. Biochim Biophys Acta. 1980 Jan 18;617(1):97–109. doi: 10.1016/0005-2760(80)90227-1. [DOI] [PubMed] [Google Scholar]
- Swank-Hill P., Needham L. K., Schnaar R. L. Carbohydrate-specific cell adhesion directly to glycosphingolipids separated on thin-layer chromatography plates. Anal Biochem. 1987 May 15;163(1):27–35. doi: 10.1016/0003-2697(87)90088-1. [DOI] [PubMed] [Google Scholar]
- Tiemeyer M., Swank-Hill P., Schnaar R. L. A membrane receptor for gangliosides is associated with central nervous system myelin. J Biol Chem. 1990 Jul 15;265(20):11990–11999. [PubMed] [Google Scholar]
- Walz G., Aruffo A., Kolanus W., Bevilacqua M., Seed B. Recognition by ELAM-1 of the sialyl-Lex determinant on myeloid and tumor cells. Science. 1990 Nov 23;250(4984):1132–1135. doi: 10.1126/science.1701275. [DOI] [PubMed] [Google Scholar]