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. 1991 Feb 15;88(4):1172–1176. doi: 10.1073/pnas.88.4.1172

Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading frame BCRFI.

P Vieira 1, R de Waal-Malefyt 1, M N Dang 1, K E Johnson 1, R Kastelein 1, D F Fiorentino 1, J E deVries 1, M G Roncarolo 1, T R Mosmann 1, K W Moore 1
PMCID: PMC50979  PMID: 1847510

Abstract

We have demonstrated the existence of human cytokine synthesis inhibitory factor (CSIF) [interleukin 10 (IL-10)]. cDNA clones encoding human IL-10 (hIL-10) were isolated from a tetanus toxin-specific human T-cell clone. Like mouse IL-10, hIL-10 exhibits strong DNA and amino acid sequence homology to an open reading frame in the Epstein-Barr virus, BCRFI. hIL-10 and the BCRFI product inhibit cytokine synthesis by activated human peripheral blood mononuclear cells and by a mouse Th1 clone. Both hIL-10 and mouse IL-10 sustain the viability of a mouse mast cell line in culture, but BCRFI lacks comparable activity in this assay, suggesting that BCRFI may have conserved only a subset of hIL-10 activities.

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