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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1991 Feb 15;88(4):1247–1250. doi: 10.1073/pnas.88.4.1247

Transposon-facilitated DNA sequencing.

M Strathmann 1, B A Hamilton 1, C A Mayeda 1, M I Simon 1, E M Meyerowitz 1, M J Palazzolo 1
PMCID: PMC50994  PMID: 1847513

Abstract

We describe here a transposon-based DNA sequencing strategy that allows the introduction of sequencing priming sites throughout a target sequence by bacterial mating. A miniplasmid was designed to select against transposon insertions into the vector. Sites of transposon insertion are mapped by the polymerase chain reaction with bacterial overnight cultures providing the templates. A small set of plasmids with transposons spaced several hundred base pairs apart can then be sequenced. Sequencing primers corresponding to the transposon ends allow sequencing in both directions. Thus, the entire sequence of both strands can be easily determined.

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Selected References

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