Skip to main content
. Author manuscript; available in PMC: 2016 Nov 9.
Published in final edited form as: Biochemistry. 2016 Jun 22;55(26):3658–3666. doi: 10.1021/acs.biochem.6b00448

Figure 4.

Figure 4

(A) In vitro ubiquitylation assay of PTEN by WWP2 using a different E2. The reaction conditions were the same as in Figure 2D except that E2 was UbcH7 instead of UbcH5c. The samples were analyzed by colloidal blue staining. (B) A single-turnover assay to measure ubiquitin transfer from WWP2 to n-PTEN and 4p-PTEN was conducted by charging WWP2 with ubiquitin and then adding PTEN after depletion of ATP using apyrase. The ubiquitylation of PTEN was analyzed by Western blotting with the anti-PTEN antibody.