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. 1991 Feb 15;88(4):1384–1388. doi: 10.1073/pnas.88.4.1384

Isolation of cDNA encoding a newly identified major allergenic protein of rye-grass pollen: intracellular targeting to the amyloplast.

M B Singh 1, T Hough 1, P Theerakulpisut 1, A Avjioglu 1, S Davies 1, P M Smith 1, P Taylor 1, R J Simpson 1, L D Ward 1, J McCluskey 1, et al.
PMCID: PMC51022  PMID: 1671715

Abstract

We have identified a major allergenic protein from rye-grass pollen, tentatively designated Lol pIb of 31kDa and with pI 9.0. A cDNA clone encoding Lol pIb has been isolated, sequenced, and characterized. Lol pIb is located mainly in the starch granules. This is a distinct allergen from Lol pI, which is located in the cytosol. Lol pIb is synthesized in pollen as a pre-allergen with a transit peptide targeting the allergen to amyloplasts. Epitope mapping of the fusion protein localized the IgE binding determinant in the C-terminal domain.

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