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. Author manuscript; available in PMC: 2016 Nov 10.
Published in final edited form as: Shock. 2016 Nov;46(5):541–548. doi: 10.1097/SHK.0000000000000648

Fig. 5. Prior amitriptyline treatment exacerbates scald-induced CD8+ T-cell depletion in thymus and spleen.

Fig. 5

Mice were pretreated with amitriptyline or saline vehicle and underwent sham or scald injury as described in the Materials and Methods. Thymocyte or splenocytes were harvested and (A) CD8+ single positive thymocytes, (B) naive CD8+ splenocyte, (C) central memory (CM) CD8+ splencoyte, and (D) effector memory CD8+ splenocyte subsets were characterized and enumerated as describe in the Materials and Methods. Sample size was 8 to 14 mice per group. Data are expressed as the mean ± SEM. Statistical comparisons were made using Student t test. *, P <0.05 as compared with scald group. Dotted line indicates sham levels. AMIT indicates amitriptyline.