Figure 4. Inhibition of Casein Kinase I (CKI) increases β-catenin transcriptional activity in MDCK quiescent monolayers, independent of mechanical strain.
Distribution of TOPdGFP at 8 hr (A), β-catenin at 8 hr (A, insets), EdU at 24 hr (C), and pY654 β-catenin at 8 hr (E) in MDCK Monolayers after No Strain or High Strain (15%) applied by the ISA and treated with either DMSO or the CKI inhibitor D4476. Quantification of percent cells TOPdGFP- (B) or EdU- (D) positive and average pY654 β-catenin intensity per pixel (F). Quantifications were from at least 3 independent experiments and, for the TOPdGFP and EdU quantifications, included analysis of 832–1364 cells per experiment. Quantifications were mean +/- SEM; unpaired t-test (B, D) or Kolmogorov-Smirnoff (F) test p values<0.05 (*), <0.01 (**), and <0.001 (***).
DOI: http://dx.doi.org/10.7554/eLife.19799.018
Figure 4—source data 1. Data used to construct graphs in Figure 4 and Figure 4—figure supplement 1.
elife-19799-fig4-data1.xlsx (38.6KB, xlsx)
DOI: 10.7554/eLife.19799.019
Figure 4—figure supplement 1. Distribution of β-catenin in monolayers treated with either DMSO or the CKI Inhibitor D4476 8 hr after the application of No Strain or High Strain (15%) using the ISA.
Scale bars: 25 μm.