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. 1991 Feb 15;88(4):1541–1545. doi: 10.1073/pnas.88.4.1541

Microglial progenitors with a high proliferative potential in the embryonic and adult mouse brain.

F Alliot 1, E Lecain 1, B Grima 1, B Pessac 1
PMCID: PMC51055  PMID: 1996355

Abstract

Single cell suspensions, prepared from brain stem, cerebellum, and forebrain parenchyma of embryonic and adult mice, were plated on monolayers of an astroglial cell line derived from a spontaneously immortalized mouse cerebellar culture, the D19 clone. A few of the brain cells adhering to the D19 monolayers were immunoreactive to the Mac-1 antibody, which labels all cells of the monocytic and granulocytic lineages. The Mac-1-positive cells proliferated vigorously and later most of them acquired the F4/80 epitope specific for macrophages and microglia cells. Studies in clonal conditions allowed development of large colonies of about 2 x 10(5) cells that expressed typical microglia markers. Bone marrow Mac-1-positive cells cocultured on D19 monolayers were also induced to proliferate, whereas peritoneal macrophages were not. D19 astrocytes express macrophage colony-stimulating factor (CSF-1) activity at a high level, and their conditioned media induced the proliferation of brain and bone marrow Mac-1-positive cells. A specific anti-CSF-1 antiserum completely blocked bone marrow macrophage progenitor proliferation and significantly reduced the multiplication of microglial precursors induced by the D19-conditioned medium. These data indicate that the embryonic and adult mouse brain parenchyma contains potential progenitors for microglial cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alliot F., Delhaye-Bouchaud N., Geffard M., Pessac B. Role of astroglial cell clones in the survival and differentiation of cerebellar embryonic neurons. Brain Res Dev Brain Res. 1988 Dec 1;44(2):247–257. doi: 10.1016/0165-3806(88)90223-4. [DOI] [PubMed] [Google Scholar]
  2. Alliot F., Pessac B. Astrocytic cell clones derived from established cultures of 8-day postnatal mouse cerebella. Brain Res. 1984 Jul 23;306(1-2):283–291. doi: 10.1016/0006-8993(84)90377-9. [DOI] [PubMed] [Google Scholar]
  3. Austyn J. M., Gordon S. F4/80, a monoclonal antibody directed specifically against the mouse macrophage. Eur J Immunol. 1981 Oct;11(10):805–815. doi: 10.1002/eji.1830111013. [DOI] [PubMed] [Google Scholar]
  4. Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bartlett P. F. Pluripotential hemopoietic stem cells in adult mouse brain. Proc Natl Acad Sci U S A. 1982 Apr;79(8):2722–2725. doi: 10.1073/pnas.79.8.2722. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Biguet N. F., Buda M., Lamouroux A., Samolyk D., Mallet J. Time course of the changes of TH mRNA in rat brain and adrenal medulla after a single injection of reserpine. EMBO J. 1986 Feb;5(2):287–291. doi: 10.1002/j.1460-2075.1986.tb04211.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Civelli O., Birnberg N., Herbert E. Detection and quantitation of pro-opiomelanocortin mRNA in pituitary and brain tissues from different species. J Biol Chem. 1982 Jun 25;257(12):6783–6787. [PubMed] [Google Scholar]
  8. Clark S. C., Kamen R. The human hematopoietic colony-stimulating factors. Science. 1987 Jun 5;236(4806):1229–1237. doi: 10.1126/science.3296190. [DOI] [PubMed] [Google Scholar]
  9. Giulian D., Baker T. J. Characterization of ameboid microglia isolated from developing mammalian brain. J Neurosci. 1986 Aug;6(8):2163–2178. doi: 10.1523/JNEUROSCI.06-08-02163.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Graeber M. B., Tetzlaff W., Streit W. J., Kreutzberg G. W. Microglial cells but not astrocytes undergo mitosis following rat facial nerve axotomy. Neurosci Lett. 1988 Mar 10;85(3):317–321. doi: 10.1016/0304-3940(88)90585-x. [DOI] [PubMed] [Google Scholar]
  11. Hetier Emmanuelle, Ayala Jésus, Bousseau Anne, Denèfle Patrice, Prochiantz Alain. Amoeboid Microglial Cells and not Astrocytes Synthesize TNF-alpha in Swiss Mouse Brain Cell Cultures. Eur J Neurosci. 1990;2(9):762–768. doi: 10.1111/j.1460-9568.1990.tb00466.x. [DOI] [PubMed] [Google Scholar]
  12. Jung-Testas I., Alliot F., Pessac B., Robel P., Baulieu E. E. Localisation immunocytochimique du cytochrome P-450scc dans les oligodendrocytes de rat en culture. C R Acad Sci III. 1989;308(6):165–170. [PubMed] [Google Scholar]
  13. Morstyn G., Burgess A. W. Hemopoietic growth factors: a review. Cancer Res. 1988 Oct 15;48(20):5624–5637. [PubMed] [Google Scholar]
  14. Perry V. H., Hume D. A., Gordon S. Immunohistochemical localization of macrophages and microglia in the adult and developing mouse brain. Neuroscience. 1985 Jun;15(2):313–326. doi: 10.1016/0306-4522(85)90215-5. [DOI] [PubMed] [Google Scholar]
  15. Richardson W. D., Pringle N., Mosley M. J., Westermark B., Dubois-Dalcq M. A role for platelet-derived growth factor in normal gliogenesis in the central nervous system. Cell. 1988 Apr 22;53(2):309–319. doi: 10.1016/0092-8674(88)90392-3. [DOI] [PubMed] [Google Scholar]
  16. Risau W., Wolburg H. Development of the blood-brain barrier. Trends Neurosci. 1990 May;13(5):174–178. doi: 10.1016/0166-2236(90)90043-a. [DOI] [PubMed] [Google Scholar]
  17. Sachs L. The molecular control of blood cell development. Science. 1987 Dec 4;238(4832):1374–1379. doi: 10.1126/science.3317831. [DOI] [PubMed] [Google Scholar]
  18. Springer T., Galfré G., Secher D. S., Milstein C. Mac-1: a macrophage differentiation antigen identified by monoclonal antibody. Eur J Immunol. 1979 Apr;9(4):301–306. doi: 10.1002/eji.1830090410. [DOI] [PubMed] [Google Scholar]

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