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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Virology. 2016 Sep 22;499:178–184. doi: 10.1016/j.virol.2016.09.005

Figure 1. E1A 1-80FH is active in transcriptional repression and interaction with p300.

Figure 1

A. Lentiviral expression constructs for E1A 1-80FH and FH-GFP. B. Luciferase reporter assays with pHER2-Luc and the lentiviral constructs. HeLa cells were co-transfected with pHER2-Luc, phRL-0 (renilla luciferase), and the lentiviral constructs as indicated, and dual luciferase assays performed as described previously (Loewenstein and Green, 2011). Data plotted represent average of three experiments. C. Interaction of E1A 1-80FH with p300. Lentiviral particles from the constructs in A were used to infect HeLa cells to establish blasticidin-resistant cells. Cell lysates were co-immunoprecipitated with Flag antibody beads, and the bound proteins eluted and examined by Western blots for p300 (upper panel) or for FH-GFP and E1A 1-80FH using HA antibody (lower panel). Lanes 2 and 3 represent two samples of eluted E1A 1-80FH complexes with high (lane 2) and low (lane 3, below detection limit in this Western blot) quantities of E1A 1-80FH.