Figure 5. Recipient Mice Lacking Fas and/or Dendritic Cells Have Higher Numbers of Activated CD4⁺ T Cells than Control Mice.

Splenic T cells from experimental mice shown in Figures 2-4 were analyzed by flow cytometry.

(A) Representative sample showing the gating of live splenocytes for CD4⁺, TCRβ⁺ cells (top) that were further sub-gated (bottom) by their activation markers (CD44^hi, CD62L^lo).

(B) Number of CD4⁺, TCRβ⁺ cells per spleen.

(C) Percent of CD4⁺, TCRβ⁺ cells that are activated (CD44^hi, CD62L^lo).

(D) Number of CD4⁺, TCRβ⁺, CD44^hi, CD62L^lo cells per spleen. ΔDC, mice that lack cDCs; Cntrl, littermate controls. Bars represent mean and SEM. **p < 0.01; ***p < 0.001 by Mann-Whitney U test. See also Figure S6.