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. 1991 Mar 15;88(6):2042–2045. doi: 10.1073/pnas.88.6.2042

Biosynthesis of the thiazole moiety of thiamin (vitamin B1) in higher plant chloroplasts.

J H Julliard 1, R Douce 1
PMCID: PMC51164  PMID: 11607160

Abstract

The chloroplast stromal proteins from spinach condense the two heterocyclic moieties of vitamin B1--4-methyl-5-(beta-hydroxyethyl)thiazole and 2-methyl-4-amino-5-hydroxymethylpyrimidine--in the presence of MgATP. We have taken advantage of this observation to study thiazole synthesis. We present data indicating that pyruvate, glyceraldehyde 3-phosphate, tyrosine, cysteine, and MgATP are required for this synthesis and that pyruvate and glyceraldehyde 3-phosphate can be replaced by 1-deoxy-D-threo-2-pentulose. The thiazole synthesis occurs at a sustained, low rate of 140 pmol per mg of protein per hr.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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