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In vivo tumor blood vessels detection. (A) In vivo contrast-enhanced ultrasound images of these tumors treated with PBS, only laser irradiation, DTSL, ITSL, DITSL without laser irradiation, ITSL+ laser or DITSL+ laser. The circles were region of interest for these treated tumors. Scale bar = 0.5 cm. (B) Immunofluorescence staining of CD31 proteins in these tumor sections at 48 h after treatments. CD31-positive cells were stained green. Scale bar = 250 μm.
