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. 1991 Mar 15;88(6):2204–2208. doi: 10.1073/pnas.88.6.2204

Identification of a T-cell-specific enhancer at the locus encoding T-cell antigen receptor gamma chain.

D J Kappes 1, C P Browne 1, S Tonegawa 1
PMCID: PMC51198  PMID: 2006158

Abstract

The gamma delta and alpha beta T-cell antigen receptor (TCR) heterodimers are expressed in a lineage-specific, mutually exclusive manner. Regulation of expression occurs at the transcriptional level. A 13-kilobase (kb) stretch of DNA encompassing variable-joining-constant segments V gamma 4-J gamma 1-C gamma 1 of the murine gamma-chain gene was examined for the presence of transcriptional enhancing elements by a transient transfection assay. DNA fragments from this region were inserted into a test plasmid containing a heterologous promoter fused to the human growth hormone gene. An 1800-base-pair (bp) fragment located 3 kb 3' to C gamma exon III was found to display enhancing activity in several T-cell lines. Maximum enhancing activity could be localized further to fragments as small as 400 bp in some cell lines. Nucleotide sequence analysis of this 400-bp segment revealed homologies to previously described core enhancer elements and to other TCR gene enhancers. The TCR gamma-chain gene enhancer is active in both gamma delta and alpha beta T cells, indicating that it is not primarily responsible for lineage-specificity of expression, but it is inactive in non-T-cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bories J. C., Loiseau P., d'Auriol L., Gontier C., Bensussan A., Degos L., Sigaux F. Regulation of transcription of the human T cell antigen receptor delta chain gene. A T lineage-specific enhancer element is located in the J delta 3-C delta intron. J Exp Med. 1990 Jan 1;171(1):75–83. doi: 10.1084/jem.171.1.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Church G. M., Ephrussi A., Gilbert W., Tonegawa S. Cell-type-specific contacts to immunoglobulin enhancers in nuclei. 1985 Feb 28-Mar 6Nature. 313(6005):798–801. doi: 10.1038/313798a0. [DOI] [PubMed] [Google Scholar]
  3. Falkner F. G., Zachau H. G. Correct transcription of an immunoglobulin kappa gene requires an upstream fragment containing conserved sequence elements. Nature. 1984 Jul 5;310(5972):71–74. doi: 10.1038/310071a0. [DOI] [PubMed] [Google Scholar]
  4. Heilig J. S., Tonegawa S. Diversity of murine gamma genes and expression in fetal and adult T lymphocytes. 1986 Aug 28-Sep 3Nature. 322(6082):836–840. doi: 10.1038/322836a0. [DOI] [PubMed] [Google Scholar]
  5. Heilig J. S., Tonegawa S. T-cell gamma gene is allelically but not isotypically excluded and is not required in known functional T-cell subsets. Proc Natl Acad Sci U S A. 1987 Nov;84(22):8070–8074. doi: 10.1073/pnas.84.22.8070. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ho I. C., Yang L. H., Morle G., Leiden J. M. A T-cell-specific transcriptional enhancer element 3' of C alpha in the human T-cell receptor alpha locus. Proc Natl Acad Sci U S A. 1989 Sep;86(17):6714–6718. doi: 10.1073/pnas.86.17.6714. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ishida I., Verbeek S., Bonneville M., Itohara S., Berns A., Tonegawa S. T-cell receptor gamma delta and gamma transgenic mice suggest a role of a gamma gene silencer in the generation of alpha beta T cells. Proc Natl Acad Sci U S A. 1990 Apr;87(8):3067–3071. doi: 10.1073/pnas.87.8.3067. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ito K., Bonneville M., Takagaki Y., Nakanishi N., Kanagawa O., Krecko E. G., Tonegawa S. Different gamma delta T-cell receptors are expressed on thymocytes at different stages of development. Proc Natl Acad Sci U S A. 1989 Jan;86(2):631–635. doi: 10.1073/pnas.86.2.631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Itohara S., Farr A. G., Lafaille J. J., Bonneville M., Takagaki Y., Haas W., Tonegawa S. Homing of a gamma delta thymocyte subset with homogeneous T-cell receptors to mucosal epithelia. Nature. 1990 Feb 22;343(6260):754–757. doi: 10.1038/343754a0. [DOI] [PubMed] [Google Scholar]
  10. Itohara S., Nakanishi N., Kanagawa O., Kubo R., Tonegawa S. Monoclonal antibodies specific to native murine T-cell receptor gamma delta: analysis of gamma delta T cells during thymic ontogeny and in peripheral lymphoid organs. Proc Natl Acad Sci U S A. 1989 Jul;86(13):5094–5098. doi: 10.1073/pnas.86.13.5094. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kranz D. M., Saito H., Heller M., Takagaki Y., Haas W., Eisen H. N., Tonegawa S. Limited diversity of the rearranged T-cell gamma gene. 1985 Feb 28-Mar 6Nature. 313(6005):752–755. doi: 10.1038/313752a0. [DOI] [PubMed] [Google Scholar]
  12. Krimpenfort P., de Jong R., Uematsu Y., Dembic Z., Ryser S., von Boehmer H., Steinmetz M., Berns A. Transcription of T cell receptor beta-chain genes is controlled by a downstream regulatory element. EMBO J. 1988 Mar;7(3):745–750. doi: 10.1002/j.1460-2075.1988.tb02871.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McDougall S., Peterson C. L., Calame K. A transcriptional enhancer 3' of C beta 2 in the T cell receptor beta locus. Science. 1988 Jul 8;241(4862):205–208. doi: 10.1126/science.2968651. [DOI] [PubMed] [Google Scholar]
  14. Pierce J. W., Lenardo M., Baltimore D. Oligonucleotide that binds nuclear factor NF-kappa B acts as a lymphoid-specific and inducible enhancer element. Proc Natl Acad Sci U S A. 1988 Mar;85(5):1482–1486. doi: 10.1073/pnas.85.5.1482. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Raulet D. H., Garman R. D., Saito H., Tonegawa S. Developmental regulation of T-cell receptor gene expression. Nature. 1985 Mar 7;314(6006):103–107. doi: 10.1038/314103a0. [DOI] [PubMed] [Google Scholar]
  16. Redondo J. M., Hata S., Brocklehurst C., Krangel M. S. A T cell-specific transcriptional enhancer within the human T cell receptor delta locus. Science. 1990 Mar 9;247(4947):1225–1229. doi: 10.1126/science.2156339. [DOI] [PubMed] [Google Scholar]
  17. Seed B., Aruffo A. Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure. Proc Natl Acad Sci U S A. 1987 May;84(10):3365–3369. doi: 10.1073/pnas.84.10.3365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Takagaki Y., Nakanishi N., Ishida I., Kanagawa O., Tonegawa S. T cell receptor-gamma and -delta genes preferentially utilized by adult thymocytes for the surface expression. J Immunol. 1989 Mar 15;142(6):2112–2121. [PubMed] [Google Scholar]
  19. Takeda J., Cheng A., Mauxion F., Nelson C. A., Newberry R. D., Sha W. C., Sen R., Loh D. Y. Functional analysis of the murine T-cell receptor beta enhancer and characteristics of its DNA-binding proteins. Mol Cell Biol. 1990 Oct;10(10):5027–5035. doi: 10.1128/mcb.10.10.5027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tonegawa S., Berns A., Bonneville M., Farr A., Ishida I., Ito K., Itohara S., Janeway C. A., Jr, Kanagawa O., Katsuki M. Diversity, development, ligands, and probable functions of gamma delta T cells. Cold Spring Harb Symp Quant Biol. 1989;54(Pt 1):31–44. doi: 10.1101/sqb.1989.054.01.005. [DOI] [PubMed] [Google Scholar]
  21. Winoto A., Baltimore D. A novel, inducible and T cell-specific enhancer located at the 3' end of the T cell receptor alpha locus. EMBO J. 1989 Mar;8(3):729–733. doi: 10.1002/j.1460-2075.1989.tb03432.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Winoto A., Baltimore D. Alpha beta lineage-specific expression of the alpha T cell receptor gene by nearby silencers. Cell. 1989 Nov 17;59(4):649–655. doi: 10.1016/0092-8674(89)90010-x. [DOI] [PubMed] [Google Scholar]

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