Figure 3.

Chromatin composition of distinct genomic loci determined by mass spectrometry. Volcano plots of proteins enriched at telomeric regions (A), major satellites (B) and minor satellites (C), respectively. Black: significantly enriched/de-enriched proteins relative to BirA*-dCas9-eGFP control cells without sgRNA. FDR = 0.01, S0 = 0.1, n = 4. (See Table S1.) (D) Overlap between proteins identified at major satellites by CasID and candidates from PICh analysis.¹⁷ (E) Overlap between proteins significantly enriched at minor and major satellite repeats. (F) Localization of ZNF512-eGFP at major satellite repeats in transiently transfected C2C12 cells. Blow-ups depict DAPI and eGFP signal of boxed regions. Conventional confocal microscopy (upper panel) shows a homogeneous and strong association of ZNF512 at heterochromatin and high-resolution microscopy (3D-SIM, lower panel) reveals a network-like structure. Scale bars: 10 µm (confocal) and 5 µm (3D-SIM). Scale bars in blow-ups: 2 µm (confocal) and 1 µm (3D-SIM).