Figure 11. The expression levels of miR-203, but not miR-542-3p, are significantly increased, associated with miR-203 promoter hypermethylation, and inversely correlated with the expression of DNMT1 and Survivin in NSCLC tumors.

(A) The expression levels of miR-203 and miR-542-3p in NSCLC tumors and their self-paired normal adjacent lung tissues (n = 20) were measured by qRT-PCR and normalized to RNU6B. (B) NSCLC tumors (T) and their self-paired normal adjacent lung tissues (N) were subjected to DNA extraction, and followed by methylation-specific PCR (MSP) and unmethylation-specific PCR (USP) analyses of miR-203 promoter. Representative results are shown. Blank control, double distilled H₂O as PCR template. Negative control, unconverted genomic DNA of normal lung tissue as PCR template. (C) Correlation analysis of methylation status of miR-203 promoter and its expression levels in NSCLC tumors (n = 20) was performed. Pearson's correlation coefficient (r) and P value are shown. (D) Correlation analyses of miR-203 or miR-542-3p expression with Survivin or DNMT1 expression in NSCLC tumors (n = 20) were performed. Pearson's correlation coefficient (r) and P values are shown.