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. 2016 May 12;7(25):37762–37772. doi: 10.18632/oncotarget.9326

Figure 1. Detection of IDO1 mRNA transcripts in mature dendritic cells.

Figure 1

Dendritic cells were generated from a healthy donor, and matured with CD40L (1 μg/ml) for 24 hours (A), IFN-γ (50 ng/ml) for 48 hours (B), or IFN-γ (50 ng/ml) plus LPS (1 μg/ml) for 48 hours (C). IDO1 mRNA transcripts were detected using the human PrimeFlow™ RNA assay. Results are expressed as percent IDO1 mRNA+ cells in the CD11c+ population (red) compared to immature dendritic cells (black: 6.9%). The experiment was repeated in 3 additional donors with similar results.