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. 2016 May 13;7(25):38036–38051. doi: 10.18632/oncotarget.9342

Figure 4. N1IC augmented gastric cancer progression through miR-151-5p.

Figure 4

A. The N1IC-expressing SC-M1/HA-N1IC and SC-M1/pcDNA3 control cells were transfected with 100 nM antagomir-151-3p (anti-miR-151-3p), antagomir-151-5p (anti-miR-151-5p), or scrambled control (−) and then seeded for MTT, colony formation, migration, and invasion assays. B. SC-M1 cells were co-transfected with N1IC-expressing construct (N1IC) or empty vector (EV) and 100 nM antagomir-151-5p or scrambled control and then seeded for MTT, colony formation, migration, and invasion assays. C. After transfection with siRNA vectors against Notch1 receptor or luciferase, SC-M1 cells were infected with adenoviruses expressing miR-151 (Ad-miR-151) or GFP (Ad-GFP) and then seeded for MTT, colony formation, migration, and invasion assays. D. After infected with adenoviruses expressing miR-151 or GFP, SC-M1 cells were treated with 50 mM DAPT and then seeded for MTT, colony formation, migration, and invasion assays. *, P <0.05; **, P <0.01; ***, P <0.001. #, P < 0.05; ##, P < 0.01; ###, P< 0.001.