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. 2016 May 20;7(25):38333–38346. doi: 10.18632/oncotarget.9518

Figure 2. Expression of EZH2 in osteosarcoma cell lines and tissues, and effects of EZH2 knockdown by siRNA in osteosarcoma cell lines.

Figure 2

(A) Expressions of EZH2 in osteosarcoma cell lines (U2OS, SAOS, KHOS, MG63), osteoblast cell lines (NHOST, HFOB, HOBC), and osteosarcoma tissues. (B and C) Western blots from A were analyzed by densitometry as described in Materials and Methods. Quantitative results of EZH2 expression for cell lines and tissues were presented as relative expression. (D) Confirmation of EZH2 expression in osteosarcoma cell lines by immunofluorescence with antibodies to EZH2 (green) and β-actin (red). Hoechst 33342 was added to counterstain the cell nucleus (blue). Green fluorescence of EZH2 protein was mainly localized in the nucleus of osteosarcoma cells. (E) siRNA knocked down EZH2 expression in osteosarcoma cells in a dose-dependent manner. (F) Confirmation of EZH2 knockdown in osteosarcoma cell lines by immunofluorescence.