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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1991 Mar 15;88(6):2366–2369. doi: 10.1073/pnas.88.6.2366

Odorant-sensitive adenylate cyclase: rapid, potent activation and desensitization in primary olfactory neuronal cultures.

G V Ronnett 1, D J Parfitt 1, L D Hester 1, S H Snyder 1
PMCID: PMC51232  PMID: 2006174

Abstract

Using primary olfactory neuronal cultures, we have demonstrated rapid, potent increases in cAMP levels and adenylate cyclase [AC; ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] activity in response to odorants. Isobutyl-methoxypyrazine is active at 1 nM. Odorant enhancement is dependent on Ca2+ concentration with maximal effects at 10-100 microM. Biphasic temporal and concentration-related effects occur with all odorants. All odorants examined elicit desensitization with AC responses abolished when odorants are reapplied immediately after removal. When reapplied 1 min after removal, odorants elicit an AC response greater than on first exposure, implying a cellular "memory" for odorants.

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Selected References

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