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. 1991 Mar 15;88(6):2422–2426. doi: 10.1073/pnas.88.6.2422

Drug-induced DNA repair: X-ray structure of a DNA-ditercalinium complex.

Q Gao 1, L D Williams 1, M Egli 1, D Rabinovich 1, S L Chen 1, G J Quigley 1, A Rich 1
PMCID: PMC51244  PMID: 2006181

Abstract

Ditercalinium is a synthetic anticancer drug that binds to DNA by bis-intercalation and activates DNA repair processes. In prokaryotes, noncovalent DNA-ditercalinium complexes are incorrectly recognized by the uvrABC repair system as covalent lesions on DNA. In eukaryotes, mitochondrial DNA is degraded by excess and futile DNA repair. Using x-ray crystallography, we have determined, to 1.7 A resolution, the three-dimensional structure of a complex of ditercalinium bound to the double-stranded DNA fragment [d(CGCG)]2. The DNA in the complex with ditercalinium is kinked (by 15 degrees) and severely unwound (by 36 degrees) with exceptionally wide major and minor grooves. Recognition of the DNA-ditercalinium complex by uvrABC in prokaryotes, and by mitochondrial DNA repair systems in eukaryotes, might be related to drug-induced distortion of the DNA helix.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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