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. 1991 Mar 15;88(6):2530–2534. doi: 10.1073/pnas.88.6.2530

Murine erythroleukemia cell line GM979 contains factors that can activate silent chromosomal human gamma-globin genes.

G Zitnik 1, P Hines 1, G Stamatoyannopoulos 1, T Papayannopoulou 1
PMCID: PMC51266  PMID: 1848707

Abstract

We introduced a normal chromosome 11 into GM979 murine erythroleukemia cells by fusing them with Epstein-Barr virus-transformed lymphocytes from a normal individual. In contrast to previous data obtained with other murine erythroleukemia cells, we detected activation of human chromosomal gamma-globin genes in GM979 cells. GM979, unlike previously used murine erythroleukemia cell lines, expresses murine embryonic globin in addition to adult globin. While all the hybrids expressed gamma- and beta-globin, they displayed a wide range of gamma-globin expression in relation to that of beta-globin. No correlation, however, was found in quantitative expression between murine embryonic globin and human gamma-globin in these hybrids, suggesting that the two globins are regulated independently, at least in this cell line. These data indicate that gamma-globin genes from normal, nonerythroid chromosomes are not irreversibly silenced, and they can be activated by a positive trans factor(s) present in GM979 cells.

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Selected References

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