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. 2016 May 26;7(26):40437–40450. doi: 10.18632/oncotarget.9624

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Copyright: © 2016 Yan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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9×10⁵ B16-OVA melanoma cells were injected intravenously in C57Bl/6J mice to establish the lung metastasis model. 3 days later, 100μg WH-OVA/OVA_257-264/WH/GA-OVA or NS was injected s.c. with CpG ODN 1826 (30μg) weekly for three times, then the lungs were processed to count the metastasis loci, and the splenocytes were isolated and re-stimulated by OVA_257-264 (10μg/mL) for 5 days. A. The scheme of tumor model, immunotherapy and ex vivo analysis. B. The effects of peptide WH-OVA_257-264 on the number of lung metastasis loci. C. Supernatant IFN-γ determined by ELISA. D. The perforin and granzyme B mRNA expression fold change. Data are presented as means ± SEM (n=5), *P<0.05, **P<0.01. Data are representative of two experiments.

9×10⁵ B16-OVA melanoma cells were injected intravenously in C57Bl/6J mice to establish the lung metastasis model. 3 days later, 100μg WH-OVA/OVA_257-264/WH/GA-OVA or NS was injected s.c. with CpG ODN 1826 (30μg) weekly for three times, then the lungs were processed to count the metastasis loci, and the splenocytes were isolated and re-stimulated by OVA_257-264 (10μg/mL) for 5 days. A. The scheme of tumor model, immunotherapy and ex vivo analysis. B. The effects of peptide WH-OVA_257-264 on the number of lung metastasis loci. C. Supernatant IFN-γ determined by ELISA. D. The perforin and granzyme B mRNA expression fold change. Data are presented as means ± SEM (n=5), *P<0.05, **P<0.01. Data are representative of two experiments.