Abstract
Alcohol is the most socially accepted addictive drug. Alcohol consumption is associated with some health problems such as neurological, cognitive, behavioral deficits, cancer, heart and liver disease. Mechanisms of alcohol-induced toxicity are presently not yet clear. One of the mechanisms underlying alcohol toxicity has to do with its interaction with amino acid-homocysteine (Hcy), which has been linked with brain neurotoxicity. Elevated homocysteine (Hcy) impairs with various physiological mechanisms in the body, especially metabolic pathways. Hcy metabolism is predominantly controlled by epigenetic regulation such as DNA methylation, histone modifications, and acetylation. An alteration in these processes leads to epigenetic modification. Therefore, in this review, we summarize the role of Hcy metabolism abnormalities in alcohol-induced toxicity with epigenetic adaptation and their influences on cerebrovascular pathology.
Keywords: DNA methylation, cystathionine-β-synthase, cystathionine-γ-lyase, cerebrovascular pathology, vascular dementia
Introduction
Alcohol intake is one of the leading threats to the health and safety of people in Western countries, affecting about 14 million people in the United States (Ron 2004). Alcohol is readily spread throughout the body in the blood stream and crosses biological membranes, which affect virtually all biological processes. Ethanol exposure causes profound damages to both the adult and developing brain. Drinking excessive amounts of alcohol (chronic) regularly for years is toxic to almost every tissue of the body. Many of the toxic effects of alcohol are due to disturbances of a wide variety of metabolic functions and organ damage. Long-term alcohol use increases the risk of various cerebrovascular disorders and different types of cancers (Agarwal and Seitz et al., 2001). Alcohol depresses the central nervous system (CNS), interfering with mood behavior, cognition and coordination.
Hyperhomocysteinemia (HHcy) is characterized by an elevated concentration of Hcy in the blood, greater than 15 μmol/L. There are different levels of HHcy: mild (15–30 μmol/L), moderate (31–100 μmol/L), or severe (>100 μmol/L). Excess production of Hcycan occur as a result of impaired metabolism due to a deficiency in cofactors (vitamin B6, B12, folate) or to genetic alteration in metabolic enzymes (methionine synthase; MS, methyltetrahydrofolate reductase; MTHFR, cystathionine-β-synthase; CBS, and cystathionine-γ-lyase; CSE). HHcy is commonly seen in about 5% to 12% of the general population and is recognized as a risk factor for cerebrovascular diseases (Okura et al., 2014; Feng et al., 2013; Clarke et al., 2000) diabetes, obesity, and hepatic steatosis (Dara and Kaplowitz et al., 2011; Kaplowitz et al., 2004). Various epidemiological and longitudinal studies suggested a causal link between Hcy and cognitive impairment (Nurk et al., 2005). This may be due to cerebrovascular as well as to direct neurotoxic mechanisms (Sachdev et al., 2005). Changes of Hcy overtime predicted the decline in memory scores in elderly subjects (Nurk et al., 2005) and several follow up studies demonstrated a positive association between Hcy at baseline and a worsening in some measures of cognitive function after several years (Ravaglia et al., 2005; McCaddon et al., 2001).
Chemistry of Homocysteine
Hcy is a naturally occurring homologue of the amino acid cysteine and differs by only an additional methylene (-CH2) group in normal physiological conditions. Hcy does not come from the diet; it is primarily synthesized from methionine (Met) through a multi-step process within the body. As previous reports suggest, excess accumulation of Hcy in the body causes cell damage and promotes vascular and microvascular disease, leading to cerebrovascular dysfunction (Dayal et al., 2004; Lominadze et al., 2006; Eren et al., 2014; Kamat et al., 2013; Muradashvili et al., 2014). This occurs because of an error in biochemical transformation in metabolic processes. It may also occur due to a lack of necessary and essential vitamins required for physiological processes within the body. The lack of these vitamin cofactors in transformation pathways can produce elevated homocysteine levels that place patients at risk for vascular disease. HHcy is associated with damage to the vascular system by a mechanism related to oxidative stress, resulting in a build-up of damaging free radicals (Tyagi et al., 2005; Kalani et al., 2014). Free radical oxygen species and other known oxidants may trigger many brain diseases including stroke and vascular dementia. These reactive chemical, oxygen, or nitrogen species generate free radicals, which can oxidize the neuronal lipid bilayer by oxidizing lipids and proteins in the vascular endothelial wall. Hcy itself can undergo auto-oxidation, thus causing the disruption of redox homeostasis and affecting the redox signaling pathways in vascular and neuronal cells (Zou et al., 2005;Perna et al., 2003; Weiss et al., 2003). Also, Hcy has been found to induce neurological dysfunction via oxidative stress (James et al., 2004; Ho et al., 2003). A large body of evidence suggests that chronic alcohol exposure is associated with impaired sulfur amino acid metabolism, and thus can alter redox stress in the brain (Worrall and Thiele et al., 2001). The proposed mechanisms by which HHcy and alcohol exposure induce injury in the brain are both underlined by vascular damage, cognitive impairment, and neurological complications that can result in a severe cerebrovascular event, such as stroke or dementia.
Metabolism of Homocysteine
The association between HHcy and cerebrovascular diseases has been suggested to be an independent risk factor for many pathological conditions, including cerebral stroke (Joseph et al., 2009). Hcy is produced as an intermediate product in Met metabolism, and its concentrations vary extensively depending on Met concentration. Hcy is metabolized by way of transsulfuration to cysteine by cystathionine-β-synthase (CBS) or re-methylated to Met through Methionine synthase (MS) or Betaine methyltransferase (BMT) (Selhub et al., 1999). Other metabolic products such as Vitamin B6, which acts as the cofactor of CBS, are essential for Hcy metabolism. In addition, folate and vitamin B12 are needed as co-substrates and cofactors for MS. It has been shown that Hcy is regulated through a series of pathways, which are dependent on B vitamins, particularly folate (Halsted et al., 2002). However, B12 deficiency, especially in elderly, is often associated with neurodegenerative disorders including vascular dementia (VAD), Parkinson’s disease (PD), multiple sclerosis, and Alzheimer’s disease (AD). Several studies have suggested that folate is very important for brain functions (Kennedy et al., 2016) and deprivation of folate induce robust increase in Hcy, oxidative stress and increased Amyloid beta (Aβ)-induced apoptosis, while folate supplementation prevented the generation of oxidative stress by Aβ (Ho et al., 2003). Treatment with the S-adenosyl hydrolase inhibitor (3-deaza adenosine), provides neuroprotection in normal, apolipoprotein E-deficient mice and in cultured neuronal cells deprived of folate and vitamin E and subjected to oxidative challenge (Tchantchou et al., 2004). In agreement with those studies, it has also been reported that intake of vitamin B12 and B6 improve brain function by lowering the levels of Hcy that exist in HHcy condition (Quadri et al., 2004).
Methylene tetrahydrofolate reductase (MTHFR) is an enzyme in Met metabolism, and it reduced activity of the MTHFR gene increases the mean plasma Hcy level, which is influenced by a single-nucleotide polymorphism in the MTHFR gene. A collective genetic variant of the MTHFR gene, also known as a key enzyme in Hcy metabolism, has been associated with an increase in Hcy levels (Delvin et al., 2004). Hcy is also involved in the metabolism of methyl groups (DNA-methylation) plays an important role in the regulation of gene expression. Therefore, it has been suggested that Hcy is an important epigenetic factor.
Homocysteine is methylated to methionine and then to S-adenosyl methionine (SAM), which is a substrate and primary methyl donor in methylation reactions. Since S-adenosyl homocysteine (SAH) is both a product and potent inhibitor of methylation reactions, the SAM/SAH ratio is considered a useful indicator for proper methylation (Tyagi et al., 2005). SAH is also the substrate for SAH hydrolase (SAHH), a reversible reaction that generates homocysteine but increases SAH when homocysteine concentrations are high. In trans-sulfuration reactions, homocysteine is a substrate for the CBS reaction (Ovechkin et al., 2006; Tyagi et al., 2007), which is facilitated by SAM to generate cystathionine and finally produces glutathione (GTH), the principal antioxidant in the body as well as in the brain (Figure. 1). Studies have revealed that drinking ethanol/alcohol is linked to elevated homocysteine levels in the plasma (Stickel et al., 2000) as well as in the liver. Reports have also suggested that consumption of ethanol along with a folate-deficient diet is associated with increased levels of homocysteine (Halsted et al., 2002). Thus, it may be concluded that increased ethanol intake and low folate diets may lead to HHcy.
Homocysteine, Alcohol and metabolic disturbances in the brain
Genetic alteration and its consequences lead to decreased expression of Hcy-metabolizing enzymes such as CBS, MTHFR and methionine synthase (MS) that leads to HHcy (Faraci and Lentz et al., 2004). Changes in plasma Hcy concentrations reflect one aspect of the metabolic consequences of methyl group deficiencies or nutrient supplementations. Folic acid supplementation spares betaine as a methyl donor. Betaine is a significant determinant of plasma Hcy, particularly in cases of folate deficiency, methionine overload, or alcohol consumption. Betaine supplementation has a lowering effect on methionine load and high Hcy levels. Increasing choline or betaine levels can reduce hypo methylation and lower Hcy levels. Folic acid supplementation also lowers the risk factor for stroke by reducing total plasma homocysteine levels. Increased levels of Hcy can cause elevated blood pressure and are considered a risk factor for recurrent strokes. There is convincing evidences about the effectiveness of vitamin-B complex supplementation in lowering the stroke risk (Gustavo et al., 2011). In particular, Hcy levels are increased in the body when metabolism of cysteine or methionine is impaired. This often occurs due to dietary deficiencies in vitamin B6, vitamin B12 and folic acid. The chronic alcoholic tends to be undernourished and is usually deficient in B vitamins. However, it remains obscure whether alcohol-dependent patients benefit from homocysteine-lowering strategies through folate, vitamin B6 and B12 supplementation, particularly in those who have low folate status (Figure. 2).
Homocysteine, Alcohol and Cerebrovascular dysfunction
Many studies suggest that heavy alcohol consumption increases the risk for cardiac disease and ischemic stroke (Hillbom et al., 1999a, b, c). It is even possible that heavy drinking and occasional drinking could trigger an ischemic stroke (Lai et al., 2012). Interestingly, moderate levels of alcohol consumption have a protective effect on coronary artery disease, cardiac disease, and hemorrhagic strokes, but alcohol consumption beyond moderate levels is risk factor for coronary artery disease, cardiac disease, hemorrhagic strokes, and chronic liver disease. An alcohol-induced stroke can occur after an acute intake of an intoxicating dose of alcohol causes a sudden marked increase of blood flow, which causes a dislodging of a thrombus present in the arterial wall. The circulatory effects of alcohol may not only dispatch emboli from the heart, but also artery-to-artery emboli from proximal arterial sources. In this context, the finding by Koehler et al., (2001) clearly suggests the strong association between total Hcy, folate intake and, alcohol consumption. Several studies suggest that brain infarction in stroke showed a positive correlation with heavy drinking of alcohol. Although chronic ethanol consumption affects plasma Hcy levels, which is associated with the risk of cerebrovascular disease (Hankey and Eikel boom et al., 1999), in consistencies with results deal with moderate alcohol consumption and increased levels of Hcy (Bleichet et al., 2001; Jacques et al., 2001), decreased levels of Hcy (Burger et al., 2004; de Bree et al., 2001), or unchanged (Ayaoriet et al., 2000) Hcy levels.
Epidemiological studies have revealed that vascular risk factors associated with diabetes are high Hcy levels and hypertension, which may play a role in the development of Alzheimer’s disease (AD). The elevated level of Hcy in the plasma is recognized as a risk factor for AD and predictive parameter for the decline in cognitive function. Ehrlich and Humpel et al., 2012 reported that homocysteine and ethanol treatments declined memory function and reduced the number of cholinergic neurons in rodents and induced blood-brain barrier leakage. Moreover, a combination of ethanol and cholesterol did not alter the memory and blood-brain barrier (BBB) leakage, but the combination of ethanol and homocysteine did promote BBB damage. These observations strongly suggest that homocysteine and alcohol in combination worsen conditions in the brain, such as cerebrovascular function (Bagheri et al., 2015). In addition, the damage accelerates the progression of brain pathologies, such as impaired cerebral blood flow, AD progression, and vascular dementia. Both AD and VD are the major forms of dementia affecting the elderly population, in which the levels of many metabolites are altered in cerebrospinal fluid (CSF) and serum (Lopamudra et al., 2013).
Alcohol and impaired brain function in association of NMDA and GABA receptor
Alcohol affects the brain’s neurons in several ways. It alters their membranes as well as their ion channels, enzymes, N-methyl-D-aspartate (NMDA) and gamma-aminobutyric acid (GABA) receptors. There are numerous reports showed ethanol action is directly associated with neurotransmitters like dopamine, serotonin, GABA, NMDA and its receptors (Fernando et al., 1997, George et al., 2007,2009,2010, Benjamin et al., 2016; Spencer et al., 2016; McClintick et al., 2016). Primary mechanism by which ethanol affects the brain is through regulation of neurotransmitters and ligands, voltage gated channels that are essential for neuronal excitability and synaptic function (Hoffman et al., 2003). By altering the function of these channels, ethanol alters neuronal properties and ultimately influences the function of the brain, and consequently behavior.
It has been found that Hcy induces neuronal cell damage by stimulating NMDA receptors as well as by producing free radicals. The neurotoxicity of Hcy via overstimulation of N-methyl-D-aspartate receptors may contribute to the pathogenesis of brain shrinkage and be linked to alcohol exposure. Short-term exposure for a few hours to ethanol produces only temporary functional changes. However, long-term ethanol exposure produces persistent neuro-adaptive changes that have serious long-term consequences to brain function. For example, chronic exposure to ethanol has been reported to increase the density of NMDA receptor binding and elevates the expression of specific NMDA receptor subunits in the brain (Hoffman et al., 2003). Similar changes in the expression of voltage-gated Ca2+ channels (VGCCs) have been reported following chronic alcohol exposure (Dolin et al., 1987). The alteration in NMDA receptors and VGCCs are thought to underlie the hyper-excitability and excitotoxicity associated with chronic alcohol use (Gulya et al., 1991; Whittington et al., 1995).
GABA receptors are probably the most common kind in the mammalian nervous system, and GABA is the primary inhibitory neurotransmitter in the brain (Tyagi et al., 2007a, b). Hcy, an excitatory amino acid, has a high binding affinity for GABA receptors, it can inhibit their activity and expression. It has been shown that alcohol mediates the rise in homocysteine, as well as the neurotransmitters GABA and glutamate and their respective receptors: GABA and NMDA (Davies, 2003). GABA reduces the activity of the signal-receiving neuron, whereas glutamate, the primary excitatory neurotransmitter, stimulates the activity of the signal receiving neuron. Alcohol has been shown to activate the GABA receptors and to inhibit the NMDA receptors (Figure. 3) and resulting in the impairment of brain functions
Alcohol and Epigenetic changes (Acetylation/Deacetylation)
Long-term alcohol exposure causes widespread changes in brain gene expression in humans and animal models (George et al., 2010, Xiang et al., 2015, McClintick et al., 2016). Many of these contribute to cellular adaptations which leads to changes in DNA and histone methylation, histone acetylation, and microRNA expression that affect expression of multiple genes in various types of brain cells (i.e., neurons and glia), that contribute to brain pathology and brain plasticity associated with alcohol abuse. Histone proteins are the second major target of epigenetic changes. The work of Ghezzi et al., 2013 explored genes for the functional alcohol tolerance by using a novel genomic epigenetic approaches of two chemically distinct alcohols. In Drosophila melanogaster, ethanol and benzyl alcohol induce mutual cross-tolerance, indicating that they share a common mechanism for producing tolerance. Ethanol, its major metabolite acetate and HHcy have been shown to induce histone H3 acetylation (Shukla et al.,2015; Fang et al.,2016). Ethanol enhanced histone H3K9 acetylation in a dose- and time-dependent manner in primary cultures of hepatocytes, without affecting H3K14 acetylation, demonstrating the specificity of ethanol in regulating covalent modifications to histone proteins. Park and colleagues (2012) reported that chronic ethanol treatment did not change global H3K9 acetylation, but did result in an increase in H3K9 acetylation. Epigenetic regulation following ethanol administration showed an increase in H3K9 acetylation and a decrease in H3K27 trimethylation marks in the brains of rodents (D’Addario et al., 2013). Histone lysine methylation is an important modification that is associated with chromatin remodeling and gene regulation and has been implicated in drug-induced neuronal plasticity mechanisms, memory formation, and cognition (Gupta et al. 2010; Maze et al. 2010; Schaefer et al., 2009; Shinkai and Tachibana et al 2011). In brief, histone acetylation and deacetylation mechanisms, especially in the brain are an important neuronal regulatory mechanism that may be involved in the development and maintenance during alcohol exposure. Deacetylation i.e histone deacetylases (HDAC) involves removal of an acetyl group on lysine residues in the N-terminal tail and on the surface of the core of histone proteins. Histone deacetylation is associated with gene silencing and alcohol regulates metabolic activity which one ways regulates gene expression. Thus, it could be suggested that (histone deacetylases inhibitor therapy may provide an alternative option to treat alcohol exposure and its underlying mechanism and causes.
Alcohol and Epigenetic changes (DNA methylation)
Elevated Hcy levels have been previously linked to altered DNA methylation levels in various diseases such as cardiovascular disease (CVD) and cerebrovascular disease. Folate or vitamin B12 based methods of lowering Hcy have had limited effects in reducing CVD events. Vitamin based therapies have limitations and have failed to reverse epigenetic changes induced by HHcy (Kalani et al. 2014). DNA methylation is a covalent modification to DNA, which occurs on cytosine residues and involves the addition of a methyl group to the C5 position (5-mc) and is catalyzed by DNA methyltransferases (DNMTs) (Bestor et al., 2000). Methylation of DNA is thought to mediate transcriptional repression via two ways: directly hindering binding of DNA-binding proteins (Klose and Bird et al., 2006) and indirectly through binding of methyl-CpG (Goll and Bestor et al., 2005). The first identified and most abundant DNMT, DNMT1 also known as a “maintenance methyltransferase” can recognize hemi-methylated DNA and perform methylation of the complementary strand. The de novo methyltransferases, DNMT3a and DNMT3b, methylate previously un-methylated cytosine’s. They are essential during development and have been implicated in synaptic plasticity in the CNS and in learning and memory mechanisms (Feng et al., 2010; Levenson et al., 2006a,b; Miller and Sweatt et al., 2007 and 2008). The demethylation of DNA is a rapidly emerging study involving a complex inter play of interdependent pathways and mechanisms (Gavin et al., 2013). Activity-dependent DNA demethylation is a dynamic process crucial to neuronal function. The work of Robert A. Philibert et al., 2012 reported that chronic alcohol intake is associated with significant changes in CpG methylation, and in particular, increased hypermethylation of CpG islands there by it causes chronic alcohol tolerance. A genome-wide DNA methylome analysis suggests that widespread alcohol-induced alterations with significant hypermethylation of many regions of chromosomes particularly those associated with molecular pathways for metabolic processes, oxidative stress, and neuronal properties of stem cells, which it causes alcohol-induced terato-genesis in nervous system (Khalid et al., 2014). Since it has been continuously reported that chronic alcohol exposure leads to a rise in plasma homocysteine levels as well as increased methylation/demethylation, therapies targeting the epigenetic machinery as well as lowering circulating Hcy may have a more valuable effect in reducing the incidence of cerebrovascular events and complications (Figure. 3).
Hyperhomocysteinemia, Alcohol and Epigenetic regulation
Elevated homocysteine levels (HHcy) in the blood of alcoholic patients have been associated with hypermethylation at the promoter of the homocysteine-induced endoplasmic reticulum protein gene, corresponding to lower mRNA expression in blood cells (Bleich et al., 2006). Hyperhomocysteinemia levels have also been implicated in hypermethylation of the alpha-synuclein (SNCA) gene promoter, which may be responsible for altered mRNA and protein expression, and was linked to alcohol cravings (Bonsch et al., 2004, 2005). Chronic alcohol consumption could be associated with altered methylation patterns of various genes acting via SAM metabolism and altering homocysteine levels. However, hypomethylation of DNA has been reported. Alternation in promoter DNA methylation levels in alcohol-dependent patients have been reported of many genes, including nerve growth factor (NGF), homocysteine induced endoplasmic reticulum protein (HERP), opiod receptor (OPRMI), dopamine transporter (DAT). Increased DNA methylation of the SNCA gene in alcoholic patients may be eventually caused in disruption of dopaminergic neurotransmission. Elevated promoter methylation in HERP results in increased rate of seizure and neurologic damage (nieratschker et al., 2013). Approaches that can detect altered transcripts and corresponding changes in methylation levels in peripheral blood samples, such as lymphocytes and mononuclear cells can be used as potential tool for the development of biomarkers (Biermann et al., 2009; Hillemacher et al., 2009;Bleich and Hillemacher et al., 2009).
Future direction
The metabolic disturbances and epigenetic processes during alcohol exposure or the combination of alcohol intake and high homocysteine levels clearly facilitate the development of cerebrovascular pathology. Cerebrovascular dysfunction, such as cerebral stroke and vascular dementia, are extensively correlated with HHcy. However, more research on the metabolic and epigenetic processes of these mechanisms, which are promising but have limitations, need to be addressed. The major issue is that the same metabolic or epigenetic changes involved in alcohol mediated high homocysteine levels are also involved in hyperhomocysteinemia alone. It needs to be explored whether this is in fact implies the same mechanisms or if there are different mechanisms in each which create cerebrovascular pathology.
The metabolic disturbances and epigenetic processes during alcohol exposure or the combination of high homocysteine levels and chronic alcohol intake clearly facilitate the development of cerebrovascular pathology. Cerebrovascular dysfunction, such as cerebral stroke and vascular dementia, are extensively correlated with hyperhomocystenemia. However, more research into the metabolic and epigenetic processes of these mechanisms, which are promising but have some limitations, needs to be addressed. Also, continued exploration of how alcohol mediates high levels of homocysteine and/or alcohol intake plus HHcy induces cerebral stroke and related cerebrovascular pathologies is needed. The major issue is that the same metabolic or epigenetic changes involved in alcohol mediated high homocysteine levels are also involved in hyperhomocysteinemia alone. It needs to be explored whether this is in fact implies the same mechanisms or if there are different mechanisms in each which create cerebrovascular pathology.
Conclusion
In the present review, we discussed the metabolic and epigenetic changes during alcohol intake and their effects on brain function altered by plasma homocysteine levels. High Hcy levels lead to alteration of the activity of DNMTs in part acetylation/deacetylation that consequently causes hypo or hyper methylation of genes. This metabolic and epigenetic alteration further affects the genes that play an important role in pathophysiology of cerebrovascular disease, such as cerebral stroke and vascular dementia. However, the role of alcohol-induced plasma Hcy levels and how this mediates metabolic and epigenetic changes are poorly explored. The authors believe that alcohol induces HHcy and that mediates metabolic and epigenetic changes. The future exploration of these mechanisms appears to be promising. Extensive studies are needed to explore the metabolic and epigenetic changes during alcohol mediated HHcy and the therapeutic mechanisms that could improve cerebrovascular pathology (Figure. 4). Much epigenetic therapeutics have been developed for other diseases, and understanding the functional relationships between epigenetic processes and the transcriptome in the alcoholic brain may lead to new molecular targets for developing medications for solving the anomalies related to alcohol consumption and alcoholism.
Acknowledgments
Financial support from National Institute of health grant HL-107640 and AR-067667 are greatly acknowledged.
Abbreviations
- Hcy
Homocysteine
- tHcy
Total Homocysteine
- CBS
Cystathionine β-synthase
- BHMT
Betaine methyltransferase
- MTHFR
Methylene tetrahydrofolate reductase
- SAH
S-adenosyl homocysteine
- SAM
S-adenosyl methionine
- AD
Alzheimer’s disease
- VD
vascular dementia
- PD
Parkinson’s disease
- GABA
Gamma-aminobutyric acid
- DNMTs
DNA methyltransferases
- MS
Methionine synthase
- CSE
Cystathionine-γ-lyase
Footnotes
Conflict of interest: The authors have declared no conflict of interest.
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