Figure 7.
Kiss1 mRNA is decreased in male but not in female mice after overnight fasting. A, Bar graphs showing uterus weight in fed and overnight fasted control (n = 5 fed, n = 6 fasted) diestrous mice. B, Bar graphs showing relative expression of Kiss1, Tac2, Pdyn, and Npy genes in mediobasal hypothalamus (MBH) of fed and overnight fasted control (n = 5 fed, n = 6 fasted) diestrous female mice. C and D, Bar graphs showing relative expression of Kiss1 (C), Tac2 (D), and Pdyn (E) genes in mediobasal hypothalamus of fed and overnight fasted Kiss1-Cre AMPKα2loxP (n = 6 fed, n = 5 fasted) and control (n = 6 fed, n = 4 fasted) male mice. D, diestrus; E, estrus; M, metestrus; P, proestrus. *, P < .05. Data in panels A and B were analyzed using a two-tailed t test, and an F test of variance showed no differences between groups. Data in panels C–E were analyzed using a two-way ANOVA and Sidak's multiple comparisons test. Only differences among treatment were noticed. No differences in the interaction or between genotypes were observed.