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. Author manuscript; available in PMC: 2016 Dec 2.
Published in final edited form as: Nature. 2016 Feb 10;530(7591):499–503. doi: 10.1038/nature16995

Extended Data Figure 3. Conformational changes in Cascade upon partial R-loop formation.

Extended Data Figure 3

Comparison of free- (PDB: 4TVX; in a darker shade) and partial R-loop bound Cascade (this study, in lighter shade). Arrows indicate the direction of the movement. The C-terminal domain (CTD) of Cse1 pivots 30° about a hinge at the Cse1 NTD-CTD interface. The amplified motion at the tip of the Cse1-CTD slides with the Cse2 dimer ~12 Å relative to the Cas7 scaffold, and protrudes upwards into the R-loop. Cse1 NTD remains in the docked position, stabilized by the clamping of Cse1 L1 loop onto the exposed tri-nucleotide motif on crRNA 5′-handle.