Functional cortical changes in relapsing–remitting multiple sclerosis at amplitude configuration: a resting-state fMRI study

Abstract

Objective

The aim of this study was to explore the amplitude of spontaneous brain activity fluctuations in patients with relapsing–remitting multiple sclerosis (RRMS) using the amplitude of low-frequency fluctuation (ALFF) method.

Methods

ALFF and SPM8 were utilized to assess alterations in regional spontaneous brain activities in patients with RRMS in comparison with healthy controls (HCs). The beta values of altered brain regions between patients with RRMS and HCs were extracted, and a receiver operating characteristic (ROC) curve was generated to calculate the sensitivities and specificities of these different brain areas for distinguishing patients with RRMS from HCs. Pearson correlation analyses were applied to assess the relationships between the beta values of altered brain regions and disease duration and Expanded Disability Status Scale (EDSS) score.

Patients and participants

A total of 18 patients with RRMS (13 females; five males) and 18 sex-, age-, and education-matched HCs (14 females; four males) were recruited for this study.

Measurements and results

Compared with HCs, patients with RRMS showed higher ALFF responses in the right fusiform gyrus (Brodmann area [BA] 37) and lower ALFF responses in the bilateral anterior cingulate cortices (BA 24 and 32), bilateral heads of the caudate nuclei, and bilateral brainstem. The ROC analysis revealed that the beta values of these abnormal brain areas showed high degrees of sensitivity and specificity for distinguishing patients with RRMS from HCs. The EDSS score showed a significant negative Pearson correlation with the beta value of the caudate head (r=−0.474, P=0.047).

Conclusion

RRMS is associated with disturbances in spontaneous regional brain activity in specific areas, and these specific abnormalities may provide important information about the neural mechanisms underlying behavioral impairment in RRMS.

Introduction

Multiple sclerosis (MS) is a neurodegenerative disorder that is characterized by motor dysfunction and disseminated axonal degeneration as well as inflammatory demyelination in the central nervous system. MS is an autoimmune demyelinating disease of the central nervous system. While demyelination is the hallmark of relapsing–remitting subtype, neurodegeneration is believed to be the hallmark of progressive forms. In addition, there is a growing consensus on a partly independent occurrence of demyelination and neurodegeneration even at early stages of MS, the link between both processes being synaptotoxicity. Typically, the extent of motor dysfunction is relative to the extent of white matter (WM) and gray matter (GM) damage.^1,2 The extensive involvement of disease-related cortical GM organization may delay and perturb syntonic intrinsic signals across cortico–cortical and cortico–subcortical circuits and underlie several nonmotor clinical manifestations of MS such as fatigue, sensorimotor deficits, and cognitive dysfunction.² However, the neurological basis of the role of WM and GM damage in these manifestations of MS remains unclear.

Brain imaging has facilitated the noninvasive exploration of MS-related pathology.^3–5 However, previous functional magnetic resonance imaging (fMRI) studies have traditionally focused on task-evoked neuronal activity. Because different performance and task patterns make it difficult to interpret biological mechanisms of disease and hinder between-study comparisons, it has been difficult to achieve a consensus regarding the nature of MS-related abnormalities in brain regions, including the anterior cingulate cortex, inferior parietal lobule, basal ganglia, thalamus, and cerebellum.^6–9

It has been reported that synchronous neuronal activity is conducive to information coordination and integration processing;¹⁰ in one study, the interruption of synchronous activity was proposed to mediate cognitive dysfunction by inducing deterioration of the efficiency and speed of information processing.¹¹ Resting-state fMRI (RS-fMRI) is a useful tool for the mechanistic investigation of brain diseases; RS-fMRI can accurately localize neural activities, does not require the use of a radioactive tracer, and has been applied to detect alterations in regional brain activity.¹² In contrast to magnetoencephalography and conventional electrophysiological methods, RS-fMRI has the capacity to map neuronal activity with high spatial resolution. Beyond detecting regional changes in brain activity, RS-fMRI also provides important information on the architecture and interconnectivity of distributed brain networks, notably by measuring patterns of spontaneous fluctuations.

Previous applications of fMRI in MS have limited analyses to specific regions of interest and were not designed to detect activation changes across the entire brain.¹³ Functional connectivity analyses using RS-fMRI permit the investigation of intrinsic brain activity across the whole brain and allow the identification of network connectivity between distant brain regions,¹⁴ especially in the default mode network (DMN).¹⁵ However, the results of abnormal functional connectivity between two remote areas do not allow for the determination of which brain area has abnormal spontaneous activity.¹⁶

The amplitude of low-frequency fluctuation (ALFF), an important component of RS-fMRI, is integrated in a low-frequency range and has been used to identify brain areas showing disturbances in blood-oxygen-level-dependent (BOLD) signal.^17,18 Previously, it was shown that ALFF index measurements were highly reproducible with scan intervals up to 1 year in chronic and stable schizophrenia patients as well as healthy controls (HCs).¹⁹ Zuo et al²⁰ also indicated a high degree of test–retest reliability for spontaneous low-frequency fluctuations within a low-frequency range and within four defined sections of narrow low-frequency bands. Indications of high test–retest reliability for the ALFF method makes this method a convenient and reliable index for RS-fMRI data analysis, with utility for the exploration of regional spontaneous brain activity abnormalities in various brain diseases.¹⁶ Recently, ALFF indices have been successfully employed for the study of sleep deprivation,^12,21 primary insomnia,¹⁶ and obstructive sleep apnea.²² However, no study to date has evaluated disturbances in spontaneous regional brain activity in patients with relapsing–remitting MS (RRMS). In the current study, we used the ALFF method as an index to explore local functional alterations of regional brain activity in patients with RRMS relative to HCs. Moreover, we examined correlations between these findings and disability scores to evaluate the relevance of ALFF indices to the clinical manifestation of RRMS.

Patients and methods

Subjects

A total of 18 patients with RRMS (five males and 13 females; mean age, 35±10.68 years; mean education, 9.22±4.21 years) diagnosed according to the McDonald’s diagnostic criteria were recruited for this study. Prior to magnetic resonance imaging (MRI) examination, all subjects underwent a thorough physical examination using the Expanded Disability Status Scale (EDSS)²³ conducted by an experienced neurologist. EDSS scores ranged from 0 to 10, where 0 indicated normal status and higher scores indicated more severe degrees of disability.

All subjects were right handed as measured by the Edinburgh inventory. The inclusion criteria for patients with RRMS were as follows: 1) mild-to-moderate disability (EDSS ≤5) to prevent the inclusion of confounding factors; 2) the absence of corticosteroid therapy or clinical relapse for at least 1 month prior to the study and during the study; 3) the absence of any sleep disorders or other psychiatric disorders according to the DSM-IV; and 4) the absence of evidence for any brain tumor, brain injury, or intracerebral hemorrhage on conventional MRI. Disease duration was recorded as the number of months from initial symptom onset date until the study MRI scan date. The mean disease duration in this study was 28.52±38.83 months. Demographic and clinical characteristics of the included patients with RRMS are presented in Table 1.

Table 1.

Demographics and clinical characteristics

Characteristics	RRMS	HCs	P-value
Sex (M/F)	5/13	4/14	0.7*
Age, years	35±10.68	35.17±10.97	0.964#
Education, years	9.22±4.21	10.72±3.95	0.286#
Disease duration, months	28.52±38.83	N/A	N/A
EDSS score	3.92±1.84	N/A	N/A

Notes:

^*χ² test;

^#independent t-test. Data are presented as mean ± standard deviation.

Abbreviations: EDSS, Expanded Disability Status Scale; F, female; HCs, healthy controls; M, male; N/A, not available; RRMS, relapsing–remitting multiple sclerosis.

A total of 18 age-, sex-, and education-matched HCs (four males and 14 females; mean age, 35.17±10.97 years; mean education, 10.72±3.95 years) were recruited for this study. HCs had no history of neurological or psychiatric disorders, alcohol or drug abuse, sleep disorders, systemic illness, brain tumor, brain injury, or intracerebral hemorrhage. The study was approved by the Human Research Ethics Committee of the Affiliated Hospital of Zunyi Medical University. The purpose, potential risks, and methods of this study were explained to all subjects, and all subjects voluntarily provided informed consent form prior to study participation.

Parameters

MRI examinations were conducted using a 3 T magnetic resonance scanner (GE, USA) with the subject in a supine position. Three-dimensional T1-weighted images were collected with a gradient-recalled echo (GRE) sequence in a sagittal position. The parameters were as follows: repetition time =3,000 ms, thickness =1.0 mm, echo time =7.8 ms, gap =0 mm, field of view =256×256 mm, acquisition matrix =256×256, and flip angle =15°. Finally, 210 functional images with 33 axial slices that covered the whole brain were collected for each subject using a GRE planar imaging pulse sequence. The parameters of the functional session were as follows: repetition time =2,000 ms, thickness =4.0 mm, echo time =30 ms, gap =0 mm, field of view =240×240 mm, and acquisition matrix =64×64, and flip angle =90°.

fMRI data analysis

To exclude defective data, all MRI data were checked using MRIcro software (www.MRIcro.com).²⁴ All MRI data were subjected to a Digital Imaging and Communications in Medicine (DICOM) form transformation. The first ten volumes of RS-fMRI images were discarded for each subject to allow for subject adaptation and account for the instability of initial MRI scanner signal. Using MATLAB2010a (Mathworks, Natick, MA, USA) and SPM8 (http://www.fil.ion.ucl.ac.uk/spm), the remaining 200 volumes were corrected for differences in slice times and were analyzed using Data Processing Assistant for RS-fMRI (DPARSFA; http://rfmri.org/DPARSF) toolbox. The time courses of head motion were collected by calculating the values of rotation (degrees) and translation (millimeter) for each volunteer. Subjects who showed >1.5 mm maximum translation in the x, y, or z planes or >1.5° of angular motion around the x, y, or z axes for any of the 200 volumes were excluded.

Three-dimensional T1-weighted images for each subject were co-registered to the mean of the realigned echo-planar imaging template and then segmented into GM, WM, and cerebrospinal fluid (CSF) using the Diffeomorphic Anatomical Registration Through Exponentiated Lie Algebra (DARTEL) segmentation. All the images were automatically transformed from the individual space into the Montreal Neurological Institute (MNI) space. Because MRI data are sensitive to micro-head motions in rotation (degrees) and translation (millimeter) in any direction (x, y, or z), the Friston 24-Parameter Model was applied to regress out head motion effects from the realigned data.^25,26 We further characterized mean frame-wise displacement (FD), which considers voxel-wise differences in motion in its derivation, as a measure of micro-head motion for each subject.

fMRI images were resampled at a resolution of 3×3×3 mm. A linear regression was performed to remove signal from the WM, GM, CSF, mean time series of all voxels across the whole brain, and head motion parameters to minimize confounding factors.²⁷ Of note, global signal was not regressed out as in previous studies^16,28–30 due to the controversial nature of this omission.^27,31 The time series for each voxel was temporally band-pass filtered (0.01–0.08 Hz) and linearly detrended to reduce low-frequency drift, cardiac noise, and physiological high-frequency respiratory artifacts. For each subject, we calculated ALFF values at each voxel. The details of the ALFF calculation are described in previous studies.^16,22

Receiver operating characteristic (ROC) curve analyses

The ROC curve method has been widely used for identifying and comparing physiological biomarkers. Sensitivity is excellent when the area under the curve (AUC) value ranges from 0.9 to 1, while sensitivity is good when the AUC value is 0.8–0.9, fair when the AUC value is 0.7–0.8, poor if the value is 0.6–0.7, and failed when the value is 0.5–0.6.^32,33 In previous studies, the ALFF index showed high degrees of sensitivity and specificity for distinguishing patients from HCs and may be useful for detecting early biological indicators of regional brain activity changes.^12,16,22

Since different brain areas may have different utility for distinguishing patients with RRMS from the HCs,²² we extracted the beta values of brain areas of interest and used them to explore whether changes in these specific areas have the ability to distinguish patients with RRMS from HCs.

Brain–behavior correlations

Based on ALFF findings, the beta values of specific brain areas in patients with RRMS were extracted, and Pearson correlation analyses were applied to assess relationships between disease duration and EDSS score.

Statistical analysis

χ² tests were used for categorical data, and two-sample t-tests were used for continuous data. The P-value <0.05 was considered to indicate statistically significant differences with two-tailed tests. All statistical analyses of demographic and clinical variables were conducted with IBM SPSS 21.0 software.

For ALFF results, two-sample t-tests were applied to analyze regional brain differences between patients with RRMS and HCs with sex, age, and education as nuisance covariates using SPM8. A corrected individual voxel P<0.01 with cluster size ≥40 was applied to assess statistical differences using an AlphaSim-corrected cluster threshold of P<0.05.

Results

ALFF differences

Compared with HCs, patients with RRMS showed higher ALFF responses in the right fusiform gyrus (Brodmann area [BA] 37) and lower ALFF responses in the bilateral anterior cingulate gyrus (BA 24 and 32), bilateral caudate head, and the cluster of the bilateral brainstem. The details of this analysis are shown in Table 2 and Figure 1.

Table 2.

Regional ALFF differences in patients with RRMS versus HCs

Conditions	Brain regions	R/L	BA	Voxel size	t-score of peak voxel	MNI coordinates (x, y, z)
RRMS > HCs	Fusiform gyrus	R	37	44	4.5473	48, −48, −21
RRMS < HCs	Pons, brainstem	L, R	N/A	43	−4.0505	6, −21, −48
RRMS < HCs	Caudate head	L, R	N/A	206	−4.4563	9, 21, 0
RRMS < HCs	Anterior cingulate gyrus	L, R	24, 32	55	−4.0923	0, 6, 45

Abbreviations: ALFF, amplitude of low-frequency fluctuation; BA, Brodmann area; HCs, healthy controls; L, left; MNI, Montreal Neurological Institute; N/A, not available; R, right; RRMS, relapsing–remitting multiple sclerosis.

Figure 1.

Different regional brain areas between patients with RRMS and HCs.

Abbreviations: HCs, healthy controls; RRMS, relapsing–remitting multiple sclerosis.

ROC findings

The mean beta values of specific clusters of interest were extracted and used for ROC curve analyses (Figure 2). ROC analyses revealed that, among ALFF areas of interest, one showed an excellent AUC value, two showed good AUC values, and one showed a fair AUC value. Further diagnostic analyses demonstrated that the mean beta values of these areas demonstrated high degrees of sensitivity and specificity. These findings indicate that regional ALFF indices could serve as good-to-excellent markers for distinguishing patients with RRMS from HCs. The details of these analyses are presented in Table 3 and Figure 3.

Figure 2.

Beta values of different ALFF brain areas.

Notes: Compared with HCs, patients with RRMS showed higher ALFF responses in the right fusiform gyrus (−0.4997±0.0448 vs −0.7858±0.0382) and lower ALFF responses in the bilateral anterior cingulate gyrus (0.0956±0.0632 vs 0.6598±0.0822), caudate head (−0.2312±0.0636 vs 0.2853±0.0842), and the cluster of the pons and brainstem (0.6776±0.1748 vs 2.5807±0.4449).

Abbreviations: ALFF, amplitude of low-frequency fluctuation; HCs, healthy controls; MS, multiple sclerosis; RRMS, relapsing–remitting MS.

Table 3.

ROC curve analysis for different ALFF areas between RRMS and HCs

Regions	ROC curve
	AUC	Sensitivity (%)	Specificity (%)	Cutoff point of mean ALFF value
Fusiform gyrus	0.886	77.8	88.9	−0.6716
Pons, brainstem	0.784	66.7	100	1.9603
Caudate head	0.877	83.3	83.3	0.02545
Anterior cingulate gyrus	0.901	88.9	77.8	0.2526

Note: All areas had high AUC values and exhibited high degrees of sensitivity and specificity.

Abbreviations: ALFF, amplitude of low-frequency fluctuation; AUC, area under the curve; HCs, healthy controls; ROC, receiver operating characteristic; RRMS, relapsing–remitting multiple sclerosis.

Figure 3.

Sensitivity and specificity of mean beta values of different brain areas.

Notes: The sensitivity and specificity of mean beta values of the right fusiform gyrus (ROI1) (A), bilateral anterior cingulate gyrus, caudate head, and the cluster of the pons and brainstem (B).

Abbreviation: ROC, receiver operating characteristic.

Pearson correlation analysis

In this study, EDSS score showed a significant negative Pearson correlation with the beta value of the caudate head (r=−0.474, P=0.047) as shown in Figure 4. There were no significant correlations between the beta values of different ALFF clusters and disease duration (P>0.05) or between EDSS score and any other ALFF cluster beta value (P>0.05).

Figure 4.

Pearson correlation between EDSS score and beta value of caudate head.

Abbreviation: EDSS, Expanded Disability Status Scale.

Discussion

In the current study, we used the RS ALFF method to investigate regional brain differences between patients with RRMS and HCs. We found that patients with RRMS showed higher ALFF responses in the right fusiform gyrus of temporal lobe and lower ALFF responses in the bilateral anterior cingulate gyrus, bilateral caudate head, and the cluster of the bilateral pons and brainstem; moreover, these observations preceded evidence of visible brain lesions on conventional MRI. Further, an ROC analysis indicated that ALFF areas of interest could effectively discriminate patients with RRMS from HCs with high degrees of sensitivity and specificity. Overall, these findings indicate that the ALFF method is useful for characterizing the neural mechanisms underlying RRMS and may be capable of detecting early biological indicators of RRMS, ie, alterations in the brain activity of relevant regions.

Previous studies have identified abnormal baseline brain activity prior to the appearance of brain lesions on T2-weighted imaging in RRMS.³⁴ Other studies comparing patients with MS to HCs have shown, in addition to abnormal regional brain activity, alterations in fractional anisotropy values, mean diffusivity values, and functional connectivity in several brain areas, including the thalamus, visual system, motor system, and areas related to advanced cognitive function.^35–42 In support of these findings, the current study identified the abovementioned differences in ALFF responses between patients with RRMS and HCs. Another previous study found smaller GM volumes in the left frontotemporal cortex, bilateral caudate nuclei, and bilateral anterior cingulate gyrus in patients with RRMS versus HCs and furthermore identified selective correlations between these brain areas and the lesion load of caudate atrophy.⁴³ In the current study, we found that EDSS score was negatively correlated with the beta value of the caudate head (r=−0.474, P=0.047). These findings provide a novel insight into the pathogenic mechanism of RRMS which, in particular, highlights the role of the caudate nucleus.

The basal ganglia play a role in both motor and advanced cognitive function. Some evidence suggests that cardiorespiratory fitness is associated with the GM volume of the basal ganglia.^44,45 In line with this hypothesis, patients with MS with higher levels of cardiorespiratory fitness were shown to have larger basal ganglia volumes, including in the caudate, putamen, and pallidum.⁴⁶ The progressive decline of anterograde memory function has been increasingly recognized as a frequent symptom in chronic MS. Previous studies have demonstrated an association between MS and poor cognitive performances,^42,47 and pediatric patients with MS were shown to have early maladaptive plasticity contributing to cognitive impairments.⁴⁸ Accordingly, increasing evidence exists for the beneficial effects of aerobic exercise training on motor and cognitive outcomes in MS and on the volumes of basal ganglia components.^49,50 In support of this concept, our study identified low ALFF responses in the bilateral caudate head in patients with RRMS, which is consistent with the previous finding that the normalized bicaudate volume was 19% lower in patients with RRMS relative to HCs.⁵¹ Overall, these findings suggest that caudate pathology underlies impairments in cognitive performance in RRMS and that cardiorespiratory fitness should be an important therapeutic goal to address these deficits.

Purely intracortical lesions represent up to 24% of the total lesion load in MS,⁵² with lesions also occurring in the cingulate gyrus⁵³ associated with axonal transection, demyelination, apoptotic loss of neurons, and dendritic transection.⁵⁴ Brainin et al⁵⁵ showed extensive lesions in the WM of the anterior cingulate gyrus in patients with MS, whereas Terroni et al⁵⁶ found that cingulate cortex dysfunction was an important factor related to working memory impairments in patients with stroke. Abnormal functional connectivity between the hippocampus and the thalamus, anterior cingulate gyrus, and prefrontal cortex has already been identified and related to impaired spatial memory function in patients with MS.⁵⁷ Accordingly, dysfunction of the bilateral anterior cingulate gyrus may underlie the impairments in working memory in patients with MS.

Conclusion

Our study demonstrated that ALFF indices in key brain regions may serve as early biological indicators of abnormal regional spontaneous brain activity in RRMS, which is observed prior to visible brain lesions on conventional MRI. The current study provided important information in order to understand the underlying neural mechanisms of RRMS pathology, and also provided insights into the relationship between RRMS and abnormal baseline brain activity and can serve as a reference for task-related fMRI studies to enable a more comprehensive understanding of the pathophysiology of RRMS. However, we did not use fatigue or other measures in the assessment; future studies should perform more measures to assess the clinical characteristics of the RRMS.