Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1991 Apr 15;88(8):3009–3013. doi: 10.1073/pnas.88.8.3009

Identification of an evolutionarily conserved, function-associated molecule on human natural killer cells.

D T Harris 1, L Jaso-Friedmann 1, R B Devlin 1, H S Koren 1, D L Evans 1
PMCID: PMC51373  PMID: 2014223

Abstract

Nonspecific cytotoxic cells (NCC) in teleost fish are analogous to human natural killer (NK) cells and spontaneously lyse a variety of transformed human cell lines sensitive to human NK cell lysis. Monoclonal antibodies (mAbs) made against these NK-like effector cells inhibited their lytic activity. These anti-NCC mAbs were examined with human NK cells for their effects. It was observed that these mAbs bound specifically to a small percentage of peripheral blood lymphocytes (5-15%) and to the majority (greater than 85%) of CD3- NK cells. The mAbs inhibited human NK cell lysis against a variety of transformed cell lines. Single-cell assays showed that the mAbs exerted their effects through inhibition of conjugate formation (recognition). However, the mAbs did not inhibit NK cell-mediated antibody-dependent cell-mediated cytotoxicity. Biochemical analysis of the NK cell molecule revealed that a dimeric structure was involved (distinct from antigen receptors on T cells). Thus, this molecule appears to be a candidate NK cell antigen receptor.

Full text

PDF
3009

Images in this article

3010Image
on p.3010
3011Image
on p.3011
3012Image
on p.3012

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Acuto O., Hussey R. E., Fitzgerald K. A., Protentis J. P., Meuer S. C., Schlossman S. F., Reinherz E. L. The human T cell receptor: appearance in ontogeny and biochemical relationship of alpha and beta subunits on IL-2 dependent clones and T cell tumors. Cell. 1983 Oct;34(3):717–726. doi: 10.1016/0092-8674(83)90528-7. [DOI] [PubMed] [Google Scholar]
  2. Baker P. E., Gillis S., Smith K. A. Monoclonal cytolytic T-cell lines. J Exp Med. 1979 Jan 1;149(1):273–278. doi: 10.1084/jem.149.1.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brenner M. B., McLean J., Dialynas D. P., Strominger J. L., Smith J. A., Owen F. L., Seidman J. G., Ip S., Rosen F., Krangel M. S. Identification of a putative second T-cell receptor. Nature. 1986 Jul 10;322(6075):145–149. doi: 10.1038/322145a0. [DOI] [PubMed] [Google Scholar]
  4. Chen A. R., McKinnon K. P., Koren H. S. Lipopolysaccharide (LPS) stimulates fresh human monocytes to lyse actinomycin D-treated WEHI-164 target cells via increased secretion of a monokine similar to tumor necrosis factor. J Immunol. 1985 Dec;135(6):3978–3987. [PubMed] [Google Scholar]
  5. Evans D. L., Carlson R. L., Graves S. S., Hogan K. T. Nonspecific cytotoxic cells in fish (Ictalurus punctatus). IV. Target cell binding and recycling capacity. Dev Comp Immunol. 1984 Fall;8(4):823–833. doi: 10.1016/0145-305x(84)90065-x. [DOI] [PubMed] [Google Scholar]
  6. Evans D. L., Graves S. S., Cobb D., Dawe D. L. Nonspecific cytotoxic cells in fish (Ictalurus punctatus). II. Parameters of target cell lysis and specificity. Dev Comp Immunol. 1984 Spring;8(2):303–312. doi: 10.1016/0145-305x(84)90037-5. [DOI] [PubMed] [Google Scholar]
  7. Evans D. L., Jaso-Friedmann L., Smith E. E., Jr, St John A., Koren H. S., Harris D. T. Identification of a putative antigen receptor on fish nonspecific cytotoxic cells with monoclonal antibodies. J Immunol. 1988 Jul 1;141(1):324–332. [PubMed] [Google Scholar]
  8. Graves S. S., Evans D. L., Cobb D., Dawe D. L. Nonspecific cytotoxic cells in fish (Ictalurus punctatus). I. Optimum requirements for target cell lysis. Dev Comp Immunol. 1984 Spring;8(2):293–302. doi: 10.1016/0145-305x(84)90036-3. [DOI] [PubMed] [Google Scholar]
  9. Harris D. T., Cianciolo G. J., Snyderman R., Argov S., Koren H. S. Inhibition of human natural killer cell activity by a synthetic peptide homologous to a conserved region in the retroviral protein, p15E. J Immunol. 1987 Feb 1;138(3):889–894. [PubMed] [Google Scholar]
  10. Harris D. T., Kozumbo W. J., Cerutti P., Cerottini J. C. Molecular mechanisms involved in T cell activation. I. Evidence for independent signal-transducing pathways in lymphokine production vs proliferation in cloned cytotoxic T lymphocytes. J Immunol. 1987 Jan 15;138(2):600–605. [PubMed] [Google Scholar]
  11. Harris D. T., Travis W. W., Koren H. S. Induction of activation antigens on human natural killer cells mediated through the Fc-gamma receptor. J Immunol. 1989 Oct 1;143(7):2401–2406. [PubMed] [Google Scholar]
  12. Herberman R. B. Natural killer (NK) cells and their possible roles in resistance against disease. Clin Immunol Rev. 1981;1(1):1–65. [PubMed] [Google Scholar]
  13. Koren H. S., Anderson S. J., Fischer D. G., Copeland C. S., Jensen P. J. Regulation of human natural killing. I. The role of monocytes, interferon, and prostaglandins. J Immunol. 1981 Nov;127(5):2007–2013. [PubMed] [Google Scholar]
  14. Kranz D. M., Tonegawa S., Eisen H. N. Attachment of an anti-receptor antibody to non-target cells renders them susceptible to lysis by a clone of cytotoxic T lymphocytes. Proc Natl Acad Sci U S A. 1984 Dec;81(24):7922–7926. doi: 10.1073/pnas.81.24.7922. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lanier L. L., Le A. M., Phillips J. H., Warner N. L., Babcock G. F. Subpopulations of human natural killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens. J Immunol. 1983 Oct;131(4):1789–1796. [PubMed] [Google Scholar]
  16. Pross H. F., Baines M. G., Rubin P., Shragge P., Patterson M. S. Spontaneous human lymphocyte-mediated cytotoxicity against tumor target cells. IX. The quantitation of natural killer cell activity. J Clin Immunol. 1981 Jan;1(1):51–63. doi: 10.1007/BF00915477. [DOI] [PubMed] [Google Scholar]
  17. Spits H., Borst J., Tax W., Capel P. J., Terhorst C., de Vries J. E. Characteristics of a monoclonal antibody (WT-31) that recognizes a common epitope on the human T cell receptor for antigen. J Immunol. 1985 Sep;135(3):1922–1928. [PubMed] [Google Scholar]
  18. Wysocki L. J., Sato V. L. "Panning" for lymphocytes: a method for cell selection. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2844–2848. doi: 10.1073/pnas.75.6.2844. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES