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. 1991 Apr 15;88(8):3116–3119. doi: 10.1073/pnas.88.8.3116

The amino acid sequence of a glutamic acid-rich protein from bovine retina as deduced from the cDNA sequence.

Y Sugimoto 1, K Yatsunami 1, M Tsujimoto 1, H G Khorana 1, A Ichikawa 1
PMCID: PMC51396  PMID: 2014230

Abstract

cDNA clones encoding a glutamic acid-rich protein were isolated from a bovine retina cDNA expression library. The cDNA sequence contained an open reading frame of 1770 base pairs encoding a protein of 590 amino acids (64,509 Da) and untranslated regions of 60 and 490 base pairs at the 5' and 3' ends, respectively. The cDNA hybridized to a 2.4-kilobase retinal mRNA. The amino acid sequence derived from the cDNA sequence contains a glutamic acid-rich domain in which 68 of 109 amino acids are glutamic acid. In addition, this domain contains four repeats of a peptide of 11 amino acids and two repeats of a peptide of 26 amino acids. A polyclonal antibody raised against a decapeptide corresponding to the undecapeptide repeat sequence reacted with a protein in an extract of bovine rod outer segments, whose molecular mass, 65 kDa, corresponded to that of the above glutamic acid-rich protein. The retinal glutamic acid-rich protein showed homology with glutamic acid-rich proteins from bovine brain and the C-terminal region of mammalian neurofilaments.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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