Table 2.

Predicted binding affinity of 16 CD4⁺ T-cell epitopes to 10 HLA DRB1 alleles.

Name	Subfamilies	Sequence	HLA-DRB1*01:01 (18.5%)	HLA-DRB1*15:01 (19.9%)	HLA-DRB1*03:01 (17.7%)	HLA-DRB1*04:01 (23.6%)	HLA-DRB1*07:01 (26.2%)	HLA-DRB1*08:01 (5.5%)	HLA-DRB1*09:01 (3.6%)	HLA-DRB1*11:01 (17.0%)	HLA-DRB1*12:01 (2.8%)	HLA-DRB1*13:01 (21.7%)
VH P60-1	IGHV3-16FR2	MNWARKAPGKGLEWV	38.88	47.97	25.24	49.18	36.71	0.89	9.48	8.05	74	3.23
VH P60-2	IGHV3-16FR2	NWARKAPGKGLEWVS	50.11	60.23	70.94	71.64	36.71	0.89	13.88	13.01	83.25	18.13
VH P60-3	IGHV3-16FR2	WARKAPGKGLEWVSG	61.86	68.73	70.94	62.65	36.71	0.89	27.91	20.27	85.79	18.13
VH P106-2	IGHV3-11FR3	KNSLYLQMNSLRAED	0.39	2.16	7.67	0.1	37.22	1.96	7.39	0.38	7.3	0.71
VH P106-4	IGHV3-11FR3	SLYLQMNSLRAEDTA	0.19	2.71	7.67	0.11	37.22	1.96	11.81	0.38	13	0.71
VH P106-8	IGHV3-11FR3	GRFTISRDNAKNSLY	65.22	34.5	2.99	3.53	52.05	23.02	43	19.36	46.16	16.46
VH P106-11	IGHV3-11FR3	QMNSLRAEDTAVYYC	20.92	40.81	5.24	6.19	63.43	5.97	61.16	20.55	50.67	4.25
VH P121-3	IGHV3-74FR3	DSVKGRFTISRDNAK	54.79	54.79	39.34	6.71	7.12	16.35	4.16	59.2	7.59	53.21
VHP121-10	IGHV3-74FR3	AKNTLYLQMNSLRAE	0.39	2.11	7.67	0.11	26.46	1.96	8.48	0.38	5.21	0.71
VHP125-13	IGHV4-30-2FR3	FSLKLSSVTAADTAV	8.78	14.94	3.66	2.04	5.34	5.55	8.2	7.1	28.52	5.9
FL1VH17	IGHV4-34FR3	SRLTISVDTSKNQFS	68.73	36.88	10.92	3.07	37.98	21.21	54.55	22.98	55.13	26.85
FL2VH3	IGHV4 FR1	PGLVKPSETLSLTCT	51.33	39.3	2.68	8.64	16.9	12.2	7.7	15.22	51.87	5.4
FL2VH4	IGHV4CDR1	KPSETLSLTCTVSGG	43.33	62.53	27.58	5.15	52	19.9	51.3	18.71	71.13	12.28
FL1VL5	IGLV1CDR1	RVTISCSGSTSNIGS	59.47	42.81	13.15	10.42	32.15	21.21	47.18	19.99	75.94	25.15
SMZL1VL1	IGLV1FR1	QSVLTQPPSASGTPG	14.48	46.26	9.46	4.02	56.29	18.52	43.5	14.02	46.16	3.87
VK P14-5	IGKV2-24FR1	QTPLSSPVTLGQPAS	46.41	33.88	22.89	9.98	47.24	14.03	61.16	16.7	76.48	21.91

Underlined: The scores of predicted binding affinity of peptides that were significantly low (<10) were underlined.