Figure 15. ACIN synapses and network.
(A) Presynaptic site (arrow) from the left ACIN onto contralateral MGIN interneurons at a dyad synapse. BTN2: bipolar tail neuron profile. (B) Dyad synapse (arrow) onto ipsilateral motor neuron MN3R and an unpaired tail interneuron (PMGN2) on the right side. (C) Synapse (arrow) from ACIN1L onto the ventral basal lamina (BL) opposite the notochord. Scale bar (a-c): 1 µm. (D) Network diagram of ACIN pathways. Layout plotted as an edge-weighted spring embedded network (Cytoscape 3.1.0: NRNB.org) based on synapse pathway strengths (see Materials and methods). Right and left neuropiles are each enclosed in a dashed line. Pathway strengths are shown as the line thickness sorted by the cumulative depth of synaptic profiles (key). The right side includes two sided PMGN interneurons and their partners. Note reciprocity of connections for ipsilateral but not contralateral partners. Thus ACINs are presynaptic to contralateral partners but not postsynaptic. Cell types abbreviated as in Figure 1—source data 1 (E) Dorsal view of reconstructed ACINs. Scale bar 10 µm.