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. 2016 Dec 6;5:e16962. doi: 10.7554/eLife.16962

Figure 4. Unpolarized, reciprocal, and serial synapses.

Figure 4.

(A) Unpolarized mixed synapse between cell 115 and cell 23 with dense-core (arrowhead) and electron-lucent (arrow) vesicles on both sides of the synaptic cleft. (B) Single section with synapse from cell 102 to cell 120 (black arrow) and a reciprocal partner synapse from cell 120 to cell 102 (red arrow). Arrowhead: membrane apposition marking a putative gap junction. (C) Serial dyad synapse (black arrow) from single neuron onto two postsynaptic targets (157 and 74), one of which is presynaptic in the same section (red arrow) at a dyad synapse onto two neurites (AMG1 and AMG2). (D) Serial monad synapse (black arrow) onto a single postsynaptic target (126) that is presynaptic at an adjacent synapse (red arrow) to a single postsynaptic target (116). Scale bars: 1 µm. (E) Series of four 60 nm sections through a single synapse. The pre- and postsynaptic cell are labelled in the top image. A clear cumulus of presynaptic vesicles is visible all images, and a clear postsynaptic density in the penultimate image (arrowhead). Scale bar: 500 nm.

DOI: http://dx.doi.org/10.7554/eLife.16962.013

Figure 4—source data 1. Comparison of synaptic complements using different parameters and exclusion criteria.
Exclusions include synapses onto the basal lamina (bm), synapses onto ependymal cells (Ep), neuromuscular junctions (Mu), synapses onto no apparent postsynaptic neuron (space), and synapses observed in fewer than two sections (>1 section). For values excluding neuromuscular junctions the neuromuscular junction values and percentages are given.
DOI: 10.7554/eLife.16962.014