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. Author manuscript; available in PMC: 2016 Dec 7.
Published in final edited form as: Biochemistry. 2016 May 31;55(23):3224–3233. doi: 10.1021/acs.biochem.6b00251

Figure 7.

Figure 7

Inhibition or activation of CP4H1 by biheteroarylcarboxylates and related analogues is dependent upon α-ketoglutarate and succinate. CP4H1 assays were performed in the presence of 2 mM ascorbate. Relative activity values are the mean (±SE) of three independent experiments and represent the ratio of CP4H activity observed in the presence and absence of a modulator. (A) Effect of added succinate and α-ketoglutarate on CP4H1 activity. (B) Effect of bipy on CP4H1 activity. (C) Effect of 25PDC on CP4H1 activity and its dependence upon α-ketoglutarate and succinate. (D, E, F) Effect of bipy55′DC (D), bipy45′DC (E), and pythiDC (F) on CP4H1 activity and their dependence upon α-ketoglutarate and succinate.