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. 1991 Apr 15;88(8):3228–3232. doi: 10.1073/pnas.88.8.3228

Complete separation of intracellular and extracellular information in NMR spectra of perfused cells by diffusion-weighted spectroscopy.

P C Van Zijl 1, C T Moonen 1, P Faustino 1, J Pekar 1, O Kaplan 1, J S Cohen 1
PMCID: PMC51419  PMID: 2014244

Abstract

A method is outlined that completely separates intracellular and extracellular information in NMR spectra of perfused cells. The technique uses diffusion weighting to exploit differences in motional properties between intra- and extracellular constituents. This allows monitoring of intracellular metabolism, and of transport of small drugs and nutrients through the cell membrane, under controlled physiological conditions. As a first example, proton spectra of drug-resistant MCF-7 human breast cancer cells are studied, and uptake of phenylalanine is monitored.

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Selected References

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