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. 1991 Apr 15;88(8):3253–3257. doi: 10.1073/pnas.88.8.3253

Complementation between Sindbis viral RNAs produces infectious particles with a bipartite genome.

U Geigenmüller-Gnirke 1, B Weiss 1, R Wright 1, S Schlesinger 1
PMCID: PMC51424  PMID: 2014249

Abstract

Sindbis virus, the type member of the alpha-viruses, is an enveloped virus containing a nonsegmented positive-strand RNA genome. We show that the nonstructural and the structural genes can function to produce infectious virus particles when they are expressed on two different RNA segments. The nonstructural genes are translated from an RNA in which the structural genes have been replaced by the chloramphenicol acetyltransferase gene [Xiong, C., Levis, R., Shen, P., Schlesinger, S., Rice, C. M. & Huang, H. V. (1989) Science 243, 1188-1191]. The structural genes are encoded in a defective-interfering RNA but are translated from a subgenomic RNA. Both segments contain the cis-acting sequences required for replication and packaging and are copackaged. This type of genome provides a model for an ancestral intermediate between alphaviruses and the multipartite positive-strand RNA viruses of plants. These different viruses show sequence similarities in their replicative proteins and are thought to have evolved from a common ancestor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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