L2 dorsal root ganglia isolated from 28-day sham or post sarcoma-injected animals (A and B, respectively) were immunostained for expression of TrkA (red, D) and P2X3 (green, E). The number of neurons that express both P2X3 and TrkA (yellow, C) represent a very small population of neurons in the dorsal root ganglia (6.8% sham, 4.7% sarcoma, F) (these dual immunostained neurons do not necessarily correspond to neuronal cell body size). Note that there was no significant change in the percentage of sensory neurons expressing either P2X3, TrkA, or co-expressing P2X3 and TrkA in animals with CIBP vs. sham control animals (F). Mean±SEM plotted.