Scientific Reports 6: Article number: 28506; 10.1038/srep28506 published online: June 24 2016; updated: December 08 2016.
In this Article the Authors incorrectly stated that they had developed a novel method termed bisulfite amplicon sequencing (BSAS). The method was reported by Masser et al. (reference 18 in the Article). Thus the following sentence (which appears verbatim in ref 18):
“By combining the benefits of bisulfite conversion, targeted amplification, tagmentation-based library construction, and NGS, we have developed a novel method termed bisulfite amplicon sequencing (BSAS) for targeted digital high accuracy quantitation of DNA methylation18”.
should read:
“In a previous study, Masser et al. developed a method termed bisulfite amplicon sequencing (BSAS) for targeted digital high accuracy quantitation of DNA methylation by combining the benefits of bisulfite conversion, targeted amplification, tagmentation-based library construction, and NGS18”.
The following sections in “Materials and Methods” failed to appropriately cite the protocols followed:
“DNA bisulfite conversion and bisulfite specific PCR”.
should read:
“DNA bisulfite conversion and bisulfite specific PCR13”.
“NGS library preparation and sequencing”.
should read:
“NGS library preparation and sequencing18”.
“Real-time PCR analysis”.
should read:
“Real-time PCR analysis13”.
An additional reference is listed below as reference 1 and should appear in the text as below:
“NGS data analysis and digital methylation quantitation”.
should read:
“NGS data analysis and digital methylation quantitation1”.
The Authors apologize for these errors.
References
- Pei L. et al. Genome-wide DNA methylation analysis reveals novel epigenetic changes in chronic lymphocytic leukemia. Epigenetics 7, 567–578 (2012). [DOI] [PMC free article] [PubMed] [Google Scholar]