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. Author manuscript; available in PMC: 2017 Dec 6.
Published in final edited form as: Structure. 2016 Dec 6;24(12):2163–2173. doi: 10.1016/j.str.2016.11.004

Figure 5. GluD2-Cbln affinity is weak regardless of oligomeric state.

Figure 5

A. Domain structure of GluD2. All domain and structural elements are drawn to scale.

B. Cbln1 bind does not bind GluD2 ectodomain (ATD+LBD) with high affinity, as observed by lack of co-elution on size-exclusion columns. Colors of chromatograms match colors of fractions as they are labeled on non-reducing gels.

C. GluD2 ATD domain can be tetramerized using a helical coiled coil zipper (tetrameric zipper, or “4Z”).

D. Cbln1 bind does not bind tetrameric GluD2 ATD-4Z with high affinity, as observed by lack of co-elution on size-exclusion columns, or by lack of heat release or uptake during ITC experiments in the presence or absence of calcium (Figure S4).