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. 1991 May 1;88(9):3647–3651. doi: 10.1073/pnas.88.9.3647

Improved serodiagnosis of hepatitis C virus infection with synthetic peptide antigen from capsid protein.

B Hosein 1, C T Fang 1, M A Popovsky 1, J Ye 1, M Zhang 1, C Y Wang 1
PMCID: PMC51509  PMID: 1850834

Abstract

Cloning and expression of hepatitis C virus have allowed the development of immunoassays to detect hepatitis C virus infection. However, currently available recombinant fusion protein C100-3 assays, based on a nonstructural protein of the virus, are limited in sensitivity, particularly for detecting acute infection. In this report seroconversion panels showed that an assay based on synthetic peptides, derived from immunodominant regions of both capsid and nonstructural proteins, accelerated hepatitis C virus antibody detection by 4-10 weeks. In screening, this enzyme immunoassay increased detection from 47% to 64% in plasmapheresis donors with elevated alanine aminotransferase levels (greater than 100 international units per liter), from 15% to 24% in anti-hepatitis B core antigen-positive blood donors, and from 28% to 42% in renal dialysis patients when compared with nonstructural peptide-based assays. The screening assay was repeatedly reactive for 27 of 2902 volunteer blood donor samples (0.93%); four sera reacted only with the capsid antigen. The peptide test distinguished true from false positive results in agreement with recombinant immunoblot assay in 96% of blood donor samples repeatably reactive on a recombinant hepatitis C virus enzyme immunoassay.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aach R. D., Szmuness W., Mosley J. W., Hollinger F. B., Kahn R. A., Stevens C. E., Edwards V. M., Werch J. Serum alanine aminotransferase of donors in relation to the risk of non-A,non-B hepatitis in recipients: the transfusion-transmitted viruses study. N Engl J Med. 1981 Apr 23;304(17):989–994. doi: 10.1056/NEJM198104233041701. [DOI] [PubMed] [Google Scholar]
  2. Alter H. J., Holland P. V., Morrow A. G., Purcell R. H., Feinstone S. M., Moritsugu Y. Clinical and serological analysis of transfusion-associated hepatitis. Lancet. 1975 Nov 1;2(7940):838–841. doi: 10.1016/s0140-6736(75)90234-2. [DOI] [PubMed] [Google Scholar]
  3. Alter H. J., Purcell R. H., Shih J. W., Melpolder J. C., Houghton M., Choo Q. L., Kuo G. Detection of antibody to hepatitis C virus in prospectively followed transfusion recipients with acute and chronic non-A, non-B hepatitis. N Engl J Med. 1989 Nov 30;321(22):1494–1500. doi: 10.1056/NEJM198911303212202. [DOI] [PubMed] [Google Scholar]
  4. Boudart D., Lucas J. C., Muller J. Y., Le Carrer D., Planchon B., Harousseau J. L. False-positive hepatitis C virus antibody tests in paraproteinaemia. Lancet. 1990 Jul 7;336(8706):63–63. doi: 10.1016/0140-6736(90)91585-x. [DOI] [PubMed] [Google Scholar]
  5. Brillanti S., Barbara L., Miglioli M., Bonino F. Hepatitis C virus: a possible cause of chronic hepatitis in alcoholics. Lancet. 1989 Dec 9;2(8676):1390–1391. doi: 10.1016/s0140-6736(89)91992-2. [DOI] [PubMed] [Google Scholar]
  6. Choo Q. L., Kuo G., Weiner A. J., Overby L. R., Bradley D. W., Houghton M. Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science. 1989 Apr 21;244(4902):359–362. doi: 10.1126/science.2523562. [DOI] [PubMed] [Google Scholar]
  7. Ebeling F., Naukkarinen R., Leikola J. Recombinant immunoblot assay for hepatitis C virus antibody as predictor of infectivity. Lancet. 1990 Apr 21;335(8695):982–983. doi: 10.1016/0140-6736(90)91055-f. [DOI] [PubMed] [Google Scholar]
  8. Hepatitis C virus antibodies in haemodialysis patients. Lancet. 1990 Jun 9;335(8702):1409–1410. [PubMed] [Google Scholar]
  9. Ikeda Y., Toda G., Hashimoto N., Kurokawa K. Antibody to superoxide dismutase, autoimmune hepatitis, and antibody tests for hepatitis C virus. Lancet. 1990 Jun 2;335(8701):1345–1346. doi: 10.1016/0140-6736(90)91228-3. [DOI] [PubMed] [Google Scholar]
  10. Kuo G., Choo Q. L., Alter H. J., Gitnick G. L., Redeker A. G., Purcell R. H., Miyamura T., Dienstag J. L., Alter M. J., Stevens C. E. An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis. Science. 1989 Apr 21;244(4902):362–364. doi: 10.1126/science.2496467. [DOI] [PubMed] [Google Scholar]
  11. McFarlane I. G., Smith H. M., Johnson P. J., Bray G. P., Vergani D., Williams R. Hepatitis C virus antibodies in chronic active hepatitis: pathogenetic factor or false-positive result? Lancet. 1990 Mar 31;335(8692):754–757. doi: 10.1016/0140-6736(90)90870-b. [DOI] [PubMed] [Google Scholar]
  12. Menitove J. E., Richards W. A., Destree M. Early US experience with anti-HCV kit in blood donors. Lancet. 1990 Jul 28;336(8709):244–245. doi: 10.1016/0140-6736(90)91767-5. [DOI] [PubMed] [Google Scholar]
  13. Miyamura T., Saito I., Katayama T., Kikuchi S., Tateda A., Houghton M., Choo Q. L., Kuo G. Detection of antibody against antigen expressed by molecularly cloned hepatitis C virus cDNA: application to diagnosis and blood screening for posttransfusion hepatitis. Proc Natl Acad Sci U S A. 1990 Feb;87(3):983–987. doi: 10.1073/pnas.87.3.983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Morgan C., Hyland C., Young I. F. Hepatitis C antibody and transaminase activities in blood donors. Lancet. 1990 Apr 14;335(8694):921–921. doi: 10.1016/0140-6736(90)90523-8. [DOI] [PubMed] [Google Scholar]
  15. Mosley J. W., Aach R. D., Hollinger F. B., Stevens C. E., Barbosa L. H., Nemo G. J., Holland P. V., Bancroft W. H., Zimmerman H. J., Kuo G. Non-A, non-B hepatitis and antibody to hepatitis C virus. JAMA. 1990 Jan 5;263(1):77–78. [PubMed] [Google Scholar]
  16. Okamoto H., Munekata E., Tsuda F., Takahashi K., Yotsumoto S., Tanaka T., Tachibana K., Akahane Y., Sugai Y., Miyakawa Y. Enzyme-linked immunosorbent assay for antibodies against the capsid protein of hepatitis C virus with a synthetic oligopeptide. Jpn J Exp Med. 1990 Aug;60(4):223–233. [PubMed] [Google Scholar]
  17. Okamoto H., Okada S., Sugiyama Y., Yotsumoto S., Tanaka T., Yoshizawa H., Tsuda F., Miyakawa Y., Mayumi M. The 5'-terminal sequence of the hepatitis C virus genome. Jpn J Exp Med. 1990 Jun;60(3):167–177. [PubMed] [Google Scholar]
  18. Polesky H. F., Hanson M. R. Transfusion-associated hepatitis C virus (non-A, non-B) infection. Arch Pathol Lab Med. 1989 Mar;113(3):232–235. [PubMed] [Google Scholar]
  19. Prince A. M., Brotman B., Grady G. F., Kuhns W. J., Hazzi C., Levine R. W., Millian S. J. Long-incubation post-transfusion hepatitis without serological evidence of exposure to hepatitis-B virus. Lancet. 1974 Aug 3;2(7875):241–246. doi: 10.1016/s0140-6736(74)91412-3. [DOI] [PubMed] [Google Scholar]
  20. Saito I., Miyamura T., Ohbayashi A., Harada H., Katayama T., Kikuchi S., Watanabe Y., Koi S., Onji M., Ohta Y. Hepatitis C virus infection is associated with the development of hepatocellular carcinoma. Proc Natl Acad Sci U S A. 1990 Sep;87(17):6547–6549. doi: 10.1073/pnas.87.17.6547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shimizu Y. K., Weiner A. J., Rosenblatt J., Wong D. C., Shapiro M., Popkin T., Houghton M., Alter H. J., Purcell R. H. Early events in hepatitis C virus infection of chimpanzees. Proc Natl Acad Sci U S A. 1990 Aug;87(16):6441–6444. doi: 10.1073/pnas.87.16.6441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Stevens C. E., Aach R. D., Hollinger F. B., Mosley J. W., Szmuness W., Kahn R., Werch J., Edwards V. Hepatitis B virus antibody in blood donors and the occurrence of non-A, non-B hepatitis in transfusion recipients. An analysis of the Transfusion-Transmitted Viruses Study. Ann Intern Med. 1984 Dec;101(6):733–738. doi: 10.7326/0003-4819-101-6-733. [DOI] [PubMed] [Google Scholar]
  23. Theilmann L., Blazek M., Goeser T., Gmelin K., Kommerell B., Fiehn W. False-positive anti-HCV tests in rheumatoid arthritis. Lancet. 1990 Jun 2;335(8701):1346–1346. doi: 10.1016/0140-6736(90)91229-4. [DOI] [PubMed] [Google Scholar]
  24. Wang J. J., Steel S., Wisniewolski R., Wang C. Y. Detection of antibodies to human T-lymphotropic virus type III by using a synthetic peptide of 21 amino acid residues corresponding to a highly antigenic segment of gp41 envelope protein. Proc Natl Acad Sci U S A. 1986 Aug;83(16):6159–6163. doi: 10.1073/pnas.83.16.6159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Weiner A. J., Kuo G., Bradley D. W., Bonino F., Saracco G., Lee C., Rosenblatt J., Choo Q. L., Houghton M. Detection of hepatitis C viral sequences in non-A, non-B hepatitis. Lancet. 1990 Jan 6;335(8680):1–3. doi: 10.1016/0140-6736(90)90134-q. [DOI] [PubMed] [Google Scholar]

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