Figure 2. nkx2.1 and arx are required for the maintenance of VM neural cell types.

(A–C) Projected confocal image stacks showing cholinergic, GABAergic, or octopaminergic neuronal populations in RNAi-treated animals. VM neural subpopulations are highlighted by white arrows. Regions of interest are represented by cartoons at the right side of the figure. Solid white lines represent the outer border of the animal, and dashed white lines mark the outer border of the CNS. Solid yellow line demarcates the border between the VM and DL brain regions. Scale bar = 50 µm. (D–F) Quantification of VM cholinergic, VM GABAergic, and octopaminergic neurons in RNAi-treated animals. Significance levels in dot plots: **p<0.01, ***p<0.001, error bars are standard deviation. CNS, Central nervous system; DL, Dorsal-lateral; VM, Ventral-medial.

DOI: http://dx.doi.org/10.7554/eLife.19735.008

Figure 2—figure supplement 1. nkx2.1 and arx are not required for maintenance of DL neural cell types.

(A–B) Projected confocal images showing cholinergic or GABAergic neurons in RNAi-treated animals. Cartoons depict region of interest for all confocal projections. Solid white lines represent outer border of the animal, dashed white lines represent the borders of the brain and solid yellow lines demarcate the plane of division between the VM and DL brain regions. Scale bars = 50 µm. (C–D) Quantification of DL cholinergic and GABAergic neurons. Graphs are dot plots, n ≥ 5. (E) Table showing number of neurons for each subtype found in RNAi-treated animals. Values are mean number of neurons ± standard deviation. DL, Dorsal-lateral; VM, Ventral-medial.

DOI: http://dx.doi.org/10.7554/eLife.19735.009

Figure 2—figure supplement 2. nkx2.1 and arx are not required for the maintenance of dopaminergic or serotonergic neurons Projected confocal images showing dopaminergic neurons or serotonergic neurons.

Dashed lines represent the outer border of the CNS. Scale bars = 50 µm. CNS, Central nervous system.

DOI: http://dx.doi.org/10.7554/eLife.19735.010