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. 1976 Jan;17(1):219–226. doi: 10.1128/jvi.17.1.219-226.1976

Induction of Type C Virions from Normal Rat Kidney Cells by 2-Deoxy-d-Glucose

Edward V Prochownik †, Sandra Panem †, Werner H Kirsten *
PMCID: PMC515406  PMID: 54442

Abstract

The sugar 2-deoxy-d-glucose (2-DG) induced the release of type C virions from an established line of normal rat kidney (NRK) cells. Within 20 h after the addition of 5 mg of 2-DG per ml to exponentially growing NRK cultures, more than 80% of the cells expressed the mammalian type C virus interspecies-specific antigen (p30) as determined by indirect cytoplasmic immunofluorescence. Maximal virion release occurred 1 to 2 days after 2-DG was added for 24 h to the growth medium, although a low level of virion production was detected as early as 2.5 h after 2-DG treatment. Studies with inhibitors of RNA synthesis indicated a requirement for de novo RNA synthesis after the addition of 2-DG. Sensitivity of NRK cells to type C virion induction was limited to a relatively short period of in vitro growth and preceded spontaneous virion release by 8 to 10 subculture generations. A model is presented for the sequential derepression of latent type C virus information in serially propagated NRK cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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